A cautionary note on pentacyanoammonioferrate use for determining L-canavanine occurrence in biological materials |
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| Authors: | G. A. Rosenthal D. L. Dahlman |
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| Affiliation: | (1) T. H. Morgan School of Biological Sciences, University of Kentucky, 40506 Lexington, Kentucky, USA;(2) Department of Entomology, University of Kentucky, 40506 Lexington, Kentucky, USA |
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| Abstract: | Summary Caution must be taken in the prevelant use of the pentacyanoammonioferrate reagent for the detection of L-canavanine in biological materias. This reagent reacts with L-histidine at neutral pH to form a pentacyanoammonioferate-histidine complex that is mistaken readily for its canavanine-containing counterpart.This work was supported by a grant from the National Science Foundation (PCM-78-20167). This is publication number 81-7-199 of the Kentucky Agriculatural Experiment Station, Lexington |
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