两种小麦的原生质体培养再生植株

报道两种基因型小麦昌乐5号和山农587胚性悬浮细胞系的建立和原生质体再生植株。小麦昌乐5号和山农587的胚性愈伤组织继代一年以后形成部分颗粒状和粉粒状结构,可用于悬浮培养。当分散好、生长快的胚性悬浮细胞系形成后,即可作为原生质体培养的材料。较长时间的悬浮培养容易使材料的胚性丧失,昌乐5号的胚性悬浮细胞系20天左右建成,其原生质体再生植株的频率为14/10~5(再生植株数/植板的原生质体数);而山农587的悬浮培养物需5个月以后才能用于原生质体培养,其原生质体再生植株的频率仅为4/10~6。

PLANT REGENERATION FROM CELL SUSPENSIONDERIVED PROTOPLASTS OF WHEAT(TRITICUM AESLIVUM L.)

Plant regeneration from embryogenic cell suspensions of wheat (cv. Chang-Le No.5 and Shannong 587)was described in this paper.The embryogenic cell suspension initiated from embryogenic calli which were induced from young embryos and subcultured for one year. The calli of var. Chang-Le were small granular in morphology and that of Shannong 587 were granular.The establishment of a fine dispersed and fast growth suspension for Chang-Le needed only 20 days, but for Shannong about five months. A short time in cell suspension subculture is beneficial to the maintenances of plant regeneration potential. The time for plant regeneration from protoplasts of Chang-Le was about three months and the frequency of differentiation into plant was 14/10~5(number of regenerated plants /number of protoplasts plated), but Shannong 587 needed six months for plant regeneration and had lower regeneration frequency(4/10~6).