野木瓜水溶性多糖的提取分离及鉴定

本文对纤维素酶法从野木瓜中提取水溶性粗多糖的工艺进行系统的研究，通过正交试验确定了最佳酶法提取条件：提取温度50℃，pH值4.0，提取时间2.5h, 酶用量3%。粗多糖经蛋白酶与Sevage法相结合脱蛋白、大孔树脂脱色及水浴透析，得到野木瓜水溶性精多糖CCP, 再经DEAE-纤维素阴离子交换层析柱，得一种洗脱组分CCP1。纸层析和Sepharose Cl-6B色谱柱分析表明CCP1为多糖纯品；用苯酚-硫酸比色法测定该多糖的糖含量为97.3%；紫外光谱分析未见蛋白质（280nm）与核酸（260nm）的特征吸收峰；红外光谱分析具有典型多糖吸收峰。结果表明，CCP1是初次从该植物中提取分离出来新的均一多糖组分。

Extraction, Separation and Identification of Water-soluble polysaccharides from Chaenomeles cathayensis

The technology for extracting crude water-soluble polysaccharides from Chaenomeles cathayensis with cellulase was studied systematically. The optimum technological conditions of enzyme assisted-extraction were obtained by orthogonal experiments: the amount of cellulase was 3% and the extracting time was 2.5 hours at 50℃ and pH4.0.The proteins in crude polysaccharides were removed by Sevage method combined with acid resistant proteinase. The pigment in polysaccharides was removed by macroporous resin and the other small molecule impurities in polysaccharides were removed by dialysis. Some fine water-soluble Chaenomeles cathayensis polysaccharides (CCP) were obtained and were further fractionated. An elution component CCP1 was got from column chromatography of DEAE-cellulose. The purity of CCP1 was examined by paper chromatography and Sepharose Cl-6B column chromatography. The polysaccharide CCP1 was measured according to the phenol-sulfuric acid assay with a saccharide content of 97.3%. The CCP1 was identified by means of UV and IR, respectively. UV spectrum showed that there was no absorption peaks of protein at 280 nm and nucleic acid at 260 nm. IR spectrum indicated that there were typical characteristic absorption peaks of polysaccharides. The result showed that CCP1 was a new homogeneous polysaccharide which was never separated from Chaenomeles cathayensis.