| 一种检测生物硫醇的荧光探针 |
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| 引用本文： | 周柯岩,杨瑜涛,李华肖,李玮.一种检测生物硫醇的荧光探针[J].河北大学学报(自然科学版),2020,40(1):27-32. |
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| 作者姓名： | 周柯岩 杨瑜涛 李华肖 李玮 |
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| 作者单位： | 河北大学化学与环境科学学院,河北保定071002;河北大学教育部药物化学与分子诊断重点实验室,河北保定071002,河北大学化学与环境科学学院,河北保定071002;河北大学教育部药物化学与分子诊断重点实验室,河北保定071002,河北大学化学与环境科学学院,河北保定071002,河北大学化学与环境科学学院,河北保定071002;河北大学教育部药物化学与分子诊断重点实验室,河北保定071002 |
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| 基金项目： | 河北省自然科学基金;国家自然科学基金;河北大学教育部药物化学与分子诊断重点实验室提升计划项目 |
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| 摘 要： | 小分子生物硫醇半胱氨酸(Cys)、同型半胱氨酸(Hcy)及谷胱甘肽(GSH)在人体中扮演着重要的角色.由于荧光探针法对硫醇的检测具有选择性好、灵敏度高、响应时间快、可实现生物体内硫醇的检测等优势,本文基于亲核加成反应机理,设计合成了一种新的选择性检测硫醇的荧光探针.该探针与硫醇作用后,荧光强度随硫醇浓度的增加逐渐增强,2 min内荧光强度可达到最大值.研究结果表明,该探针在生物成像方面有潜在的应用价值.
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| 关 键 词： | 荧光探针 生物硫醇 生物成像 |
| 收稿时间： | 2019-04-27 |
A fluorescent probe for detection of biothiols |
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| ZHOU Keyan,YANG Yutao,LI huaxiao,LI Wei.A fluorescent probe for detection of biothiols[J].Journal of Hebei University (Natural Science Edition),2020,40(1):27-32. |
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| Authors: | ZHOU Keyan YANG Yutao LI huaxiao LI Wei |
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| Institution: | 1. College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China; 2. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding 071002, China |
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| Abstract: | Biothiols, cysteine(Cys)and homocysteine(Hcy)and glutathione(GSH)play key roles in human body. Owing to the advantages of fluorescence probe method, such as high sensitivity, excellent selectivity, fast response time and the ability to detect thiols in vivo, a new fluorescence probe for the selective detection of biothiols based on the nucleophilic addition mechanism was designed and synthesized in this paper. Upon addition of biothiols, the fluorescence intensity increased with the increase of thiol concentration and reached its maximum within 2 min. The results showed that the probe has potential application in bioimaging. |
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| Keywords: | fluorescent probe biothiols bioimaging |
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