| Isolation and Characterization of a New Subspecies of Aeromonas salmonicida （ Aeromonas salmonicida subsp, flounderacida subsp. nov. ） from Stone Flounder（ Kareius bicoloratus L. ） |
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| 引用本文： | 张晓君,Zhan Wenbin,Chen Cuizhen,Fang Hai.Isolation and Characterization of a New Subspecies of Aeromonas salmonicida （ Aeromonas salmonicida subsp, flounderacida subsp. nov. ） from Stone Flounder（ Kareius bicoloratus L. ）[J].高技术通讯(英文版),2004,10(4):87-93. |
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| 作者姓名： | 张晓君 Zhan Wenbin Chen Cuizhen Fang Hai |
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| 作者单位： | [1]LaboratoryofPathologyandImmunologyofAquaticAnimals,3heKeyLaboratoryofMariculture,MinistryofEducation,OceanUniversityofChina,Qingdao266003,P.R.China [2]DepartmentofAnimalScience,HebeiNormalUniversityofScienceandTechnology,Qinhuangdao066600,P.R.China |
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| 基金项目： | ThisworkwassupportedbyNational863Project(granted 2 0 0 1AA62 80 2 0 )，NSFC (granted 3 0 2 710 16)，ProjectsundertheMajorStateBasicResearchDevelopmentProgram (grantedG19990 12 0 0 2 )，NaturalScienceFoundationofHebei (granted 3 0 2 43 1) . |
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| 摘 要： | Biological properties were studied to appropriate pathogenic bacteria which were isolated from diseased (or dead) stone flounder (Kareius bicoloratus L. ) which expressed bacterial septicaemia, including morphological characteristics, colony characteristics, physiological and biochemical characteristics and serum homology of isolates, the results showed that the isolates belonged to a new subspecies of A. salmonicida. In addition, the representative strains have been re-checked and detected the mol% G C ratio of the DNA by China Center for Type Culture Collection (CCTCC), the examined strains were also regarded as a new subspecies of A. salmonicida, and designated as Aeromonas salmonicida subsp, flounderac/da subsp, nov. by its isolated fish (Kareius bicoloratus ). Molecular identification of analysis of the nucleotide sequence of the 16S rRNA gene were applied, the results showed high similarity (99%) with the 16S rRNA gene of Aeromonas salmonicida from GenBank database. Cluster analysis of phylogenetic tree revealed that the representative strain formed separately bootstrap-supported cluster.
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| 关 键 词： | 石鲽 生物特征 病源体 RNA 比目鱼 |
Isolation and Characterization of a New Subspecies of Aeromonas salmonicida (Aeromonas salmonicida subsp.Flounderacida subsp.Nov.) from Stone Flounder(Kareius bicoloratus L.) |
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| Institution: | Laboratory of Pathology and Immunology of Aquatic Animals, The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P.R.China;Department of Animal Science, Hebei Normal University of Science and Technology, Qinh;Laboratory of Pathology and Immunology of Aquatic Animals, The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P.R.China;Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066600, P.R.China |
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| Abstract: | Biological properties were studied to appropriate pathogenic bacteria which were isolated from di-seased (or dead) stone flounder (Kareius bicoloratus L.) which expressed bacterial septicaemia, including morphological characteristics, colony characteristics, physiological and biochemical characteristics and serum homology of isolates, the results showed that the isolates belonged to a new subspecies of A.salmonicida. In addition, the representative strains have been re-checked and detected the mol% G+C ratio of the DNA by China Center for Type Culture Collection (CCTCC), the examined strains were also regarded as a new subspecies of A.salmonicida, and designated as Aeromonas salmonicida subsp. flounderacida subsp.nov.by its isolated fish (Kareius bicoloratus). Molecular identification of analysis of the nucleotide sequence of the 16S rRNA gene were applied, the results showed high similarity (99%) with the 16S rRNA gene of Aeromonas salmonicida from GenBank database. Cluster analysis of phylogenetic tree revealed that the representative strain formed separately bootstrap-supported cluster. |
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| Keywords: | stone flounder (Kareius bicoloratus L ) aeromonas salmonicida subsp flounderacida subsp nov biological properties 16S rRNA |
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