| 溶氧控制对黄色短杆菌YILW合成L-异亮氨酸的影响 |
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| 引用本文： | 白亚磊,徐庆阳,谢希贤,陈宁. 溶氧控制对黄色短杆菌YILW合成L-异亮氨酸的影响[J]. 天津科技大学学报, 2011, 26(1): 5-9,30 |
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| 作者姓名： | 白亚磊 徐庆阳 谢希贤 陈宁 |
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| 作者单位： | 工业微生物教育部重点实验室,天津科技大学生物工程学院,天津,300457 |
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| 基金项目： | 天津市应用基础及前沿技术研究计划项目(08JCZDJC15400) |
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| 摘 要： | 对黄色短杆菌YILW合成L-异亮氨酸的发酵溶氧条件进行了探索,构建了该菌合成上L-异亮氨酸的代谢网络和代谢流平衡模型.在30 L发酵罐中考察了不同溶氧浓度下L-异亮氨酸发酵过程.研究结果表明:高溶氧浓度有利于菌体生长,15%溶氧浓度下产酸速率高且维持的时间长,有利于L-异亮氨酸的积累.为此提出了分段控氧模式:在菌体生长...
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| 关 键 词： | L-异亮氨酸 溶氧 分段控氧模式 代谢流分析 |
Effect of Dissolved Oxygen Control on L-isoleucine Synthesis by Brevibacterium flavum YIL W |
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| BAI Ya-lei,XU Qing-yang,XIE Xi-xian,CHEN Ning. Effect of Dissolved Oxygen Control on L-isoleucine Synthesis by Brevibacterium flavum YIL W[J]. Journal of Tianjin University of Science & Technology, 2011, 26(1): 5-9,30 |
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| Authors: | BAI Ya-lei XU Qing-yang XIE Xi-xian CHEN Ning |
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| Affiliation: | BAI Ya-lei,XU Qing-yang,XIE Xi-xian,CHEN Ning(Key Laboratory of Industrial Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China) |
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| Abstract: | The condition of dissolved oxygen of L-isoleucine synthesis in Brevibacterium flavum YILW was exploreda,nd the metabolic networks and metabolic flux balance model were constructed.The fermentation processes were studied on the condition of different dissolved oxygen in 30 L fermenter.The results show that high dissolved oxygen leads to bacterium growth.Higher production ratio of L-isoleucine maintains a long time under the condition of 15% dissolved oxygen.Thus the grading-controlling mode of dissolved oxyg... |
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| Keywords: | L-isoleucine dissolved oxygen phase oxygen control model metabolic flux analysis |
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