速率法、丙酮酸氧化酶法与微板赖氏法检测ALT的对比试验

目的：为血站筛查ALT选择较准确可靠的试验方法提供试验依据．方法：由内蒙古通辽市中心血站提供的63份ALT含量不同的血浆标本进行速率法（简称S法）、丙酮酸氧化酶法（简称B法）与微板赖氏法（简称L法）比较．结果：L法、S法、B法的中值定值血清（接近血站的判断标准），板内变异系数依次为7．03％、3．98％、2．47％；L法、S法、B法的中值定值血清板间变异系数依次为7．86％、5．75％、5．50％．结论：微板丙酮酸氧化酶法可以作为血站筛查ALT的方法．

Contrast Test on Velocity Method, Pyruvate Oxidase Method and Micro-plate Reitman Frankel Assay Detecting ALT

Objective. To provide the proof for the accurate and reliable method to detect ALT in blood bank. Methods： The contents of ALT in 63 plasma samples supplied by Tongliao city central blood bank were detected via velocity method （S method）, pyruvate oxidase method （ B method ） and micro-plate Reitman Frankel assay （ L method ）. Results ： Intragroup coefficient of variation for the midvalue of evaluated serum （ approximate to the criterion of the blood bank ） for L method, S method and B method was 7.03 %, 3.98 % and 2.47 % respectively. Intergroup coefficient of variation for the midvalue of evaluated serum for three methods was 7.86 % , 5.75 % and 5.50 % respectively. Conclusion. Micro-plate pyruvate oxidase method can be applied as a screening method for ALT in blood bank through the analysis on the three methods.