| 氨基移换实验中纸层析方法的改进研究 |
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| 引用本文： | 刘美艳,营孙阳,张健,朱爱华.氨基移换实验中纸层析方法的改进研究[J].实验科学与技术,2012,10(6):21-22,37. |
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| 作者姓名： | 刘美艳 营孙阳 张健 朱爱华 |
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| 作者单位： | 江苏师范大学生命科学学院,江苏徐州,221116 |
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| 基金项目： | 江苏省重点专业类建设，江苏师范大学重点专业类建设，江苏师范大学实验室建设与管理研究 |
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| 摘 要： | 由于传统氨基移换实验中的展层剂水饱和苯酚是有毒物质,寻找低毒或无毒的展层剂是十分必要的。文章通过对展层剂的种类、配比、展层时间及染色方法的研究,确定了以正丁醇∶甲酸(88)∶水为15∶3∶2的展层剂,展层时间为1.5h,以展层剂为溶剂的0.3茚三酮为染色液,并使展层与染色同步进行。
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| 关 键 词： | 氨基移换 纸层析 展层剂 染色 |
Improvement Study Chromatography on the Experiment of Amino Transfer Method of Paper and Exchange |
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| LIU Mei-yan,YING Sun-yang,ZHANG Jian,ZHU Ai-hua.Improvement Study Chromatography on the Experiment of Amino Transfer Method of Paper and Exchange[J].Experiment Science & Technology,2012,10(6):21-22,37. |
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| Authors: | LIU Mei-yan YING Sun-yang ZHANG Jian ZHU Ai-hua |
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| Institution: | (School of Life Science, Jiansu Normal University, Xuzhou 221116, China) |
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| Abstract: | The traditional chromatographic solution in the experiment of paper chromatography of amino transfer and exchange was saturation phenol which was toxic. It is necessary to find chromatographic solution which was low toxic or non-toxic. This paper investigates the chromatographic solutions, proportion, chromatographic time and staining method. The results showed that the suitable chromatographic solution was n-butanol, formic acid and water, and corresponding proportion is 15： 3： 2. The suitable chromatographic time was 1.5h. The staining solution was 0. 3 % ninhydrin which solved with chromatographic solution, and chromatography and staining were synchronous. |
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| Keywords: | amino transfer and exchange paper chromatography chromatographic solution staining |
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