ddy小鼠替代大鼠的经济简便的抗焦虑药筛选改进模型--间氯苯哌嗪诱导的小鼠明暗箱焦虑模型

目的建立一个部分模拟临床病理的简便易得、经济有效的用于筛选和研制抗焦虑药的模型.方法用间氯苯哌嗪(mCPP)诱导产生焦虑,观察ddy小鼠和ICR小鼠在明暗箱的行为表现.结果 mCPP在SC 1-4mg/kg的剂量下即可显著降低ddy小鼠在明箱的活动次数,而对暗箱的活动次数影响不显著;1mg/kg与4mg/kg组比较,在明箱的活动次数无显著差异;mCPP在SC 2-10mg/kg的剂量下对ICR小鼠在明箱的的活动次数影响不太显著,只在10mg/kg剂量下才显著;而对暗箱的活动次数却影响较大,在2mg/kg的剂量下即可显著降低活动次数.用安定对该模型进行验证,发现只用较小的样本量即可得出显著结果.结论用ddy小鼠可以取代Wistar大鼠建立mCPP诱导焦虑的明暗箱模型,在样本量较小的情况下即可得到有效结果.所以是一个能够克服大鼠实验缺点的、简便易得且经济有效的用于筛选和研制抗焦虑药的良好模型.但用mCPP诱导的焦虑模型对小鼠的种属有选择性,ICR小鼠不适于上述模型.

mCPP-induced Anxiety Model in Light-dark Box in ddy Mice -- A New Economic and Simple Method for Screening Anxiolytic Activity

Objective To develop a new partially clinical mimic, economic and simple anxiety model for screening and studying anxiolytic model. Method observing the behavior activity of DDY and ICR mice in Light_Dark box after SC mCPP to induce anxiety. Result It can be significantly decrease the activity of ddy mice in light box after sc 1-4mg/kg, but the activity in dark box is not significantly changed. There is no significant difference in the light box between the 1mg/kg and the 4mg/kg group. mCPP has little effect on the activity of ICR mice in the light box, but it can significantly decrease the activity in the dark box between 2-10mg/kg. It is easy to prove the anxiolytic effect of Diazepam only in a small sample by using this model. Conclusion It is easy to get the effective result by using only small sample in the Light_Dark box after mCPP_induced anxiety in ddy mice, so we suppose it is partially clinical mimic, economic and simple anxiety model for screening and studying anxiolytic model.