| 紫外分光光度技术测定植物细胞中H2O2的含量 |
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| 引用本文： | 夏金婵,苗雨晨,宋纯鹏,苗琛. 紫外分光光度技术测定植物细胞中H2O2的含量[J]. 四川大学学报(自然科学版), 2005, 42(6): 1263-1265 |
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| 作者姓名： | 夏金婵 苗雨晨 宋纯鹏 苗琛 |
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| 作者单位： | 河南大学生命科学学院,河南省植物逆境重点实验室,河南,开封,475001 |
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| 基金项目： | 国家自然科学基金（30470986） |
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| 摘 要： | 利用H2O2在24Onm处有吸收光谱的特性，建立了快速测定植物活细胞产生H2O2的方法．同时利用该技术进一步定量检测了ABA诱导蚕豆气孔保卫细胞H2O2的产生．
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| 关 键 词： | 分光光度技术 H2O2定量测定 ABA 蚕豆 |
| 文章编号： | 0490-6756（2005）06-1263-03 |
| 收稿时间： | 2005-05-08 |
| 修稿时间： | 2005-05-08 |
A New Method about Determining H2O2 Content in Plant Cells by Spectrophotometry |
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| XIA Jin-chan,MIAO Yu-chen,SONG Chun-peng,MIAO Chen. A New Method about Determining H2O2 Content in Plant Cells by Spectrophotometry[J]. Journal of Sichuan University (Natural Science Edition), 2005, 42(6): 1263-1265 |
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| Authors: | XIA Jin-chan MIAO Yu-chen SONG Chun-peng MIAO Chen |
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| Affiliation: | Department of Biology, Henan University, Henan Kaifeng 475001, China |
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| Abstract: | It was reported that a method for a rapid determining H2O2content in epidermis strip of of Vicia faba by spectrophotometry has been established through the absorbanee at 24O nm. In addition, this result provided a new quantitative determination method for H2O2 involved ABA-induced stomatal movement in Vicia faba. |
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| Keywords: | spectrophotometry quantitative determination of H2O2 ABA Vicia faba |
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