不同植物生长调节物质对华黄芪愈伤组织形成及器官发生的效应

以华黄芪（ＡｓｔｒａｇａｌｕｓｃｈｉｎｅｎｓｉｓＬ．）为材料，ＭＳ为基本培养基，设计不同浓度的ＮＡＡ、２，４－Ｄ、６－ＢＡ、ＫＴ等单因子试验和不同比值的细胞分裂素／生长素组合试验，探讨植物生长调节物质对华黄芪愈伤组织形成及器官发生的效应．结果表明：在愈伤组织形成过程中，单因子试验以５．０ｍｇ／ＬＮＡＡ、０．５ｍｇ／Ｌ２，４－Ｄ、１．０ｍｇ／Ｌ６－ＢＡ、２０ｍｇ／ＬＫＴ诱导效果较好，诱导频率可达１００％，且生长势好．组合试验比单因子试验能更有效地诱导愈伤组织形成．在器官发生过程中，单独使用生长素类有不定根形成，ＮＡＡ效果较好，２，４－Ｄ次之．单独使用细胞分裂素类有不定芽的形成，６－ＢＡ效果较好，ＫＴ次之．组合试验中，细胞分裂素／生长素比值大时有利于不定芽的形成，反之则有利于不定根的形成

EFFECTS OF PLANT GROWTH REGULATORS ON CALLUS FORMATION AND ORGANOGENESIS OF ASTRAGALUS CHINENSIS L

The study on the effects of plant growth regulators on callus formation and organogenesis of Astragalus chinensis L revealed that: In the course of callus formation, the MS media with 5 0 mg/L NAA,0 5 mg/L 2,4 D,1 0 mg/L 6 BA,2 0 mg/L KT were better,all the induction frequencies of callus got to 100%,and the calli were growing well From the calli,the MS medium added 2 0 mg/L NAA was optimal for induction of adventitious roots,its induction frequencies were 46 7%,53 7%,33 3%,respectively.