浅论提高叶绿体色素提取和分离纸谱法的实验效果

本文从掌握实验全过程的整体性观点出发,论述了注意实验材料的筛选和处理,严守选择提取溶剂种类的原则和用量规律,研磨过程要充分而迅速,过滤要稳妥而充分,认真纯化色素收集液,层折液、滤纸、点样划线要得当,分离色带操作要把握好时间,分离出的各部分色素的色带名称、颜色、宽窄位置要标注准确和清晰,注意色带的科学保存等各个环节,是提高该实验效果的基本保证。

DISCUSSION ON EXPERIMENT EFFECTS OF PAPER CHROMATOGRAPHY ON IMPROVING EXTRACTION AND SEPARATION OF CHLOROPHYLL PIGMENT

Considering the integrated view of mastering the whole experimental pro-cess,the paper discussed all the conditions for screen and treatment of experiment materi-al; the rule of selecting kind and quantity of extraction solvent; the full and swift grind, full and stable filtration; the purification of pigment collection; adequate stratified analysis solvent, filter paper, dropping sample and scratching line; controlling the best time of separating chromatogram; having an exact, clean, clear, paper chromatogram and mark-ing its name,colour, width, position. These are the essential guarantee of the experimen-tal effects.