| 紫色甘薯茎尖脱毒与快繁及试管苗移植技术研究 |
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| 引用本文： | 王碧琴,盖安俊. 紫色甘薯茎尖脱毒与快繁及试管苗移植技术研究[J]. 江西科学, 2010, 28(2): 196-198,202 |
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| 作者姓名： | 王碧琴 盖安俊 |
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| 作者单位： | 1. 江西省科学院生物资源研究所,江西,南昌,330029
2. 南昌工程学院,江西,南昌,330099 |
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| 摘 要： | 以紫色甘薯块根为外植体在70%酒精浸渍2 min,0.1%HgCl2溶液消毒12 min无菌下培养催芽,3-5 d获取无菌芽苗。茎尖分生组织（0.2-0.4 mm）含1-2片叶原基在MS＋6-BA 0.1 mg/L＋NAA 0.05 mg/L下诱导培养成不定芽苗,继而分段在MS＋6-BA0.25-1.0 mg/L＋NAA 0.2-0.3 mg/L培养基增殖快繁最佳。培养不定芽试管苗3-5 cm接种在1/2MS＋IAA0.25 mg/L＋IBA0.25 mg/L培养基5 d生根率100%。试管苗移植试验结果显示脱毒苗比种薯苗产量提高,藤蔓茂盛长势强,结薯整齐薯块大,薯皮光滑,颜色鲜艳,抗逆性增强。
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| 关 键 词： | 紫色甘薯块根 茎尖分生组织 增殖快繁 试管苗移植 |
Studies on the Rapid Propagation of Virus-free Purple-colored Sweet Potato and the Transplantation of Tube Plantlets |
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| WANG Bi-qin,GAI An-jun. Studies on the Rapid Propagation of Virus-free Purple-colored Sweet Potato and the Transplantation of Tube Plantlets[J]. Jiangxi Science, 2010, 28(2): 196-198,202 |
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| Authors: | WANG Bi-qin GAI An-jun |
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| Affiliation: | 1.Institute of Biological Resources/a>;Jiangxi Academy of Science/a>;Jiangxi Nanchang 300029 PRC/a>;2.Nanchang Institute of Technology/a>;Jiangxi Nanchang 300099 PRC |
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| Abstract: | The tubers of purple-colored sweet potato were sterilized in 70% alcohol for 2 min and 0.1% HgCl2 for 12 min for accelerating germination in vitro.After 3~5 d 0.2~0.4 mm length of shoot apical meristems with 1~2 leaf primordiums were cut from the sterilized buds germinated from the tubers and cultured in MS+6-BA 0.1 mg/L+NAA 0.05 mg/L for adventitious buds germinating.The cuttings of the adventitious buds propagated in MS+6-BA 0.25~0.1mg/L+NAA 0.2~0.3 mg/L.The shoots of 3~5 cm length were explanted in 1/2 M... |
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| Keywords: | Tuber of purple-colored sweet potato Shoot apical meristem Rapid propagation Transplant of test tube plantlet |
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