| 旱芹人工种子的研制 |
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| 引用本文： | 倪德祥 沈大棱. 旱芹人工种子的研制[J]. 复旦学报(自然科学版), 1991, 30(4): 368-374 |
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| 作者姓名： | 倪德祥 沈大棱 |
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| 作者单位： | 复旦学报环境与资源生物学系(倪德祥，蒋如敏，张蓓，岑益群)，复旦学报遗传学研究所(沈大稜)，复旦学报材料科学系(纪才圭) |
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| 摘 要： |
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| 关 键 词： | 旱芹 人工种子 种子 芹菜 人工方式 |
PRODUCTION OF ARTIFICIAL SEEDS OF Apium graveolens |
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| Ni Dexiang,Jiang Rumin,Zhang Bei,Cen Yiqun,. PRODUCTION OF ARTIFICIAL SEEDS OF Apium graveolens[J]. Journal of Fudan University(Natural Science), 1991, 30(4): 368-374 |
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| Authors: | Ni Dexiang Jiang Rumin Zhang Bei Cen Yiqun |
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| Abstract: | This paper reports on the somatic embryogeny and the production of artificialseed of Apium graveolens. Somatic embryos were produced from callus in a hormone-free MS medium. The callus was produced from leaves and leafstalks in MS mediumcontaining 1 mg/L 2.4-D and 0.5 mg/L KT. The somatic embryos were mixed inMS modified medium (used as artificial endosperm) containing 2%(w/v) sodiumalginate and dropped into a solution of 1%(w/v) calcium chloride to from artificialseeds. The hardness of the seeds was 2.0 kg/capsule, and their conversion frequency88.5%~90.0%. The seeds were successfully transferred to the open in flowerpotsand grew after the germination under sterile conditions. The factors, that affectedthe conversion frequency, shch as the degree of the development of the somaticembryos, temperature, and light were also studied. |
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| Keywords: | Apium graveolens seeds artificial artificial seeds. MS medium. |
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