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Cloning and expression of the Chinese wheat mosaic virus RNA2 coat protein readthrough and 19 ku cysteinerich domains and localization of these proteins
Authors:Lei Xu  Jianping Chen  Rong Ye  Xiuyun Zhao  Shanqing Yu
Institution:(1) Microbiology Department, Fudan University, 200433 Shanghai, China;(2) Virology Laboratory, Zhejiang Academy of Agricultural Sciences, 200433 Hangzhou, China
Abstract:The 5′-terminal (RTn) and 3′-terminal (RTc) halves of the coat protein readthrough domain and the 19 ku cysteine-rich protein of Chinese wheat mosaic virus (CWMV) were amplified by RT-PCR, cloned and expressed in E. coli. Antisera and monoclonal antibodies against these proteins were prepared by immunising these purified proteins to mice. Detection of RTn, RTc and 19 ku proteins in CWMV infected wheat sap and leaf tissue indicated that the RTn and RTc proteins were distributed on the surface of virus particles whereas the 19 ku protein was in the cytoplasm of the infected wheat cells.
Keywords:Chinese wheat mosaic virus  coat protein readthrough domain  cysteine-rich domain  prokaryotic expression  localisation
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