应用RAPD鉴定红菇组织分离菌株的探索试验

用组织分离技术从野生红菇（Ｒｕｓｓｕｌａ ｓｐ．）子实体中中分离得３个菌株。用随机扩增多态性ＤＮＡ（ＲＡＰＤ）对红菇子实体和分离菌株的ＤＮＡ多样性进行分析，以确定３个分离菌株与子实体之间的亲缘关系。实验使用１６个随机引物检测４０多个位点，计算它们之间的相似性。实验结果表明３个分离菌株和红菇子实体之间的ＤＮＡ相似性非常高，红菇子实体和对照菌株「凤尾菇（Ｐｌｅｕｒｏｔｕｓ ｓａｊｏｒ－ｃａｊｕ）和金针

Identification of Russula's Isolates with RAPD

Three strains were obtained from the sporocarp of wild Russula using tissue culture isolation.Random amplified polymorphic DNA(RAPD) was used to approach genetic diversity of wild Russula and three strains, so as to decide the relation between three strains and sporocarp. Over forty genetic sites were checked by using sixteen random primers, and the genetic similarity between three strains and Russula sporecarp was studied.The result showed that they were greatly similar and the similarity gap of DNA between Russula sporocarp and the checked strains showed the interspecies distance. A preliminary conclusion can be drawn from the experiment that the three strains were isolates of Russula.