| siRNA抑制BCR—ABL基因诱导K562细胞凋亡的初探 |
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| 引用本文： | 黄国韦,王晟. siRNA抑制BCR—ABL基因诱导K562细胞凋亡的初探[J]. 新余高专学报, 2009, 14(2): 82-84 |
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| 作者姓名： | 黄国韦 王晟 |
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| 作者单位： | 1. 汕头大学医学院,病理学教研室,广东,汕头,515041
2. 汕头大学医学院,精神卫生中心,广东,汕头,515041 |
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| 摘 要： | 目的：通过mRNA结构预测软件预测出BCR—ABLmRNA二级结构，未形成二级结构的区域作为靶序列。方法：针对BCR—ABLmRNA二级结构中的单链区域设计四对siRNA，通过Lipotap脂质体作为转染载体去转染K562细胞。结果：所设计的四对siRNA其中有一对siRNA转染K562细胞后，K562细胞出现凋亡现象。结论：通过该方法有针对性的设计siRNA，避免了把mRNA的二级结构区域作为靶序列，提高了所设计siRNA的效果。
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| 关 键 词： | BCR—ABL mRNA siRNA 细胞凋亡 |
Exploration into the restraint of siRNA on the apoptosis of K562 cells induce by BCR-ABL genes |
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| HUANG Guo-wei,WANG Sheng. Exploration into the restraint of siRNA on the apoptosis of K562 cells induce by BCR-ABL genes[J]. Journal of XinYu College, 2009, 14(2): 82-84 |
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| Authors: | HUANG Guo-wei WANG Sheng |
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| Affiliation: | (Shantou University, Shantou 515041 China) |
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| Abstract: | Purpose ： to predict BCR - ABLmRNA second - level structure through mRNA structure prediction software and with the area which has not formed second - level structure as target sequence. Method ： according to the single chain area in BCR - ABLmRNA sec- ond - level structure, designing four pairs of siRNA and transferring IC562 cells with Liptotap liposome as transfer vector. Result： among four pairs of siRNA, only one pair caused the apoptosis of K562 cells after transferring them. Conclusion： by designing siRNA, avoiding taking the second - level structure of mRNA as the target sequence and improving the effect of sinRNA. |
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| Keywords: | BCR-ABL mRNA siRNA |
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