| 链霉菌转化阿魏酸生产香草酸 |
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| 引用本文： | 许美玲,王芳.链霉菌转化阿魏酸生产香草酸[J].北京化工大学学报(自然科学版),2007,34(Z2):99-102. |
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| 作者姓名： | 许美玲 王芳 |
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| 作者单位： | 北京化工大学 1生命科学与技术学院;
2北京市生物加工重点实验室， 北京 100029 |
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| 基金项目： | 国家自然科学基金
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北京市科技计划 |
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| 摘 要： | 选育一株链霉菌Streptomyces 2036，能将阿魏酸转化为香草酸。通过培养基和发酵条件的优化，确定较适培养条件为：葡萄糖5g/L，酵母粉5g/L；最佳发酵条件为：pH5.0，摇瓶装液量50mL/250mL，转速130r/min，发酵温度30℃。在该条件下，链霉菌可在转接培养16h后添加阿魏酸溶液，经12h可将1g/L的底物阿魏酸转化为0.261g/L的香草酸，摩尔转化率为30.2%。
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| 关 键 词： | 链霉菌 微生物转化 阿魏酸 香草酸 链霉菌 转化率 阿魏酸 生产 香草酸 ferulic acid biotransformation 摩尔 酸溶液 后添加 培养条件 转接 发酵温度 转速 装液量 摇瓶 最佳 酵母粉 葡萄糖 优化 |
| 收稿时间： | 2007-04-09 |
| 修稿时间： | 2007年4月9日 |
Streptomyces biotransformation of ferulic acid to vanillic acid |
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| XU MeiLing,WANG Fang.Streptomyces biotransformation of ferulic acid to vanillic acid[J].Journal of Beijing University of Chemical Technology,2007,34(Z2):99-102. |
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| Authors: | XU MeiLing WANG Fang |
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| Institution: | 1. College of Life Science and Technology; 2. Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | Streptomyces 2036 was screened for its ability to transform ferulic acid to vanillic acid. The optimal medium was found to contain glucose (5g/L) and yeast extract (5g/L). Cultures in 250mL flasks containing 50mL medium were brought to pH 5.0 and incubated by shaking at 150r/min at 30℃. Under these conditions, Streptomyces 2036 produced vanillic acid when ferulic acid was added 16h after inoculation. After a further 12h, 1g/L ferulic acid was transformed to 0.261g/L vanillic acid with a molar yield of 30.2%. It was shown that intracellular enzyme was mainly responsible for catalyzing the transformation of ferulic acid to vanillic acid. |
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| Keywords: | Streptomyces2036 biotransformation ferulic acid vanillic acid |
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