| 基于功能性CuS纳米粒子共振光散射法测定蛋白质 |
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| 引用本文： | 张明翠,张德兴,汪乐余,夏婷婷,董玲,王伦.基于功能性CuS纳米粒子共振光散射法测定蛋白质[J].安徽师范大学学报(自然科学版),2004,27(3):295-298. |
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| 作者姓名： | 张明翠 张德兴 汪乐余 夏婷婷 董玲 王伦 |
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| 作者单位： | 安徽师范大学,化学与材料科学学院,安徽,芜湖,241000;安徽师范大学,化学与材料科学学院,安徽,芜湖,241000;安徽师范大学,化学与材料科学学院,安徽,芜湖,241000;安徽师范大学,化学与材料科学学院,安徽,芜湖,241000;安徽师范大学,化学与材料科学学院,安徽,芜湖,241000;安徽师范大学,化学与材料科学学院,安徽,芜湖,241000 |
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| 基金项目： | 国家自然科学基金
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安徽师范大学校科研和教改项目
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安徽省自然科学基金 |
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| 摘 要： | 基于蛋白质的加入使功能性CuS纳米粒子的共振光散射明显增强,建立了定量测定蛋白质的新方法.最佳实验条件下,对人血清球蛋白的线性范围为0.1-7.0 μg ml-1,检出限为13 ng ml-1,对人血清白蛋白的线性范围为0.25-11.0 μg ml-1,检出限为26 ng ml-1.该方法成功用于人血清样品的测定,方法简单、灵敏、稳定、选择性好.
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| 关 键 词： | Resonance light scattering globulin Nano-CuS 功能性 纳米粒子 共振光散射法 测定 蛋白质 Functionalized Proteins Determination Technique Light Scattering good selectivity stability chemical system determination method characterized high sensitivity Human dynamic range |
Resonance Light Scattering Technique for the Determination of Proteins with Functionalized Nano-CuS |
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| Abstract.Resonance Light Scattering Technique for the Determination of Proteins with Functionalized Nano-CuS[J].Journal of Anhui Normal University(Natural Science Edition),2004,27(3):295-298. |
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| Authors: | Abstract |
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| Abstract: | Based on the strong enhancement effect of proteins on resonance light scattering of functionalized nano-CuS at 383 nm, a new direct quantitative determination method for proteins in blood serum samples is established. Under optimal conditions, the enhanced RLS intensity is in proportion to the globulin concentration in the range 0.1-7.0 (g ml~(-1). The limit of detection is 13 ng ml~(-1). And the dynamic range for human serum albumin is 0.25-11.0 (g ml~(-1) with detection limit of 26 ng ml~(-1). Human blood serum samples were determined satisfactorily. This method is characterized by high sensitivity, simplicity, good selectivity and stability of the chemical system. |
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| Keywords: | Resonance light scattering globulin Nano-CuS |
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