Spectrofluorimetric determination of magnesium (II) with 7-(8-Hydroxy-3, 6-disulfonaphthylazo)-8-hydroxyquinoline-5-sulfonic acid |
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Authors: | Zhang Xian Wang Hong Zhang Hua-shan |
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Affiliation: | (1) College of Chemistry and Molecular Science, Wuhan University, 430072 Wuhan, China |
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Abstract: | A new fluorescent reagent, 7-(8-hydroxy-3, 6-disulfonaphthylazo)-8-hydroxyquinoline-5-sulfonic acid (HDNHQ) for the determination of magnesium has been developed. It reacted with magnesium to form a 1∶1 fluorescent complex withλ ex/λ em immediately at room temperature in ammonia-ammonium chloride buffer (pH 10.7). A linear relationship was obtained in the magnesium concentration range of 0–160 ng·mL−1 with the detection limit of 0.04 ng·mL−1. The proposed method was simple, rapid and sensitive. It has been successfully applied to the determination of trace magnesium in blood serum with recoveries of 103.75% and 98.16%, respectively. Foundation item: Supported by the Zi-Qiang Foundation of Wuhan University Biography: Zhang Xian (1975-), female, Ph. D candidant, research direction: organic reagent synthesis and analysis. |
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Keywords: | 7-(8-Hydroxy-3 6-disulfonaphthylazo)-8-hydroxyquinoline-5-sulfonic acid magnesium spectrofluorimetry blood serum |
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