Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing |
| |
Authors: | SuoWei Wu Yang Xiao Xu Zheng YingFan Cai Jaroslav Dole?el BingHua Liu Li Yang MeiFang Song Peng Zhou Yang Zhou FanHua Meng ShanHong Wang HongWei Liu HuQu Zhai JianPing Yang |
| |
Institution: | 1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China 2. Graduate School, Chinese Academy of Agricultural Sciences, Beijing, 100081, China 3. College of Bio-information, Chongqing University of Posts and Telecommunication, Chongqing, 400065, China 4. Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Olomouc, CZ-77200, Czech Republic 5. Institute of Vegetable Research, Shandong Academy of Agricultural Sciences, Jinan, 250100, China
|
| |
Abstract: | The large genome size (~17000 Mb) and complicated DNA structures of common wheat (Triticum aestivum) hamper its genome sequencing. By means of flow cytometry, systematic investigations on individual chromosome sorting have been carried out to construct chromosome-specific bacterial artificial chromosome (BAC) libraries since the 1980s. Several wheat chromosome-specific BAC libraries, such as chromosome 3B, three D genome chromosomes (1D, 4D and 6D), and the short arm of chromosome 1B, have been developed, and the physical map of chromosome 3B was established in 2008. The same chromosome-based strategy is being employed by the International Wheat Genome Sequencing Consortium (IWGSC) to establish the physical maps of the other 20 common wheat chromosomes (cv. Chinese Spring). Several projects on wheat genome sequencing are currently underway. The availability of new sequencing technologies provides new choices for sequencing of gene space of the wheat genome. The applications of flow-sorted chromosomes in wheat genome studies present some examples to analyze the complex genomes of cereals. |
| |
Keywords: | Triticum aestivum flow cytogenetics chromosome sorting physical map wheat genome sequencing |
本文献已被 CNKI SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
|