甲醛滴定法测定牛血清白蛋白的酶解度

建立了一种测定蛋白质酶解度的方法，介绍了甲醛滴定法测定酶解度的四个关键环节：酚酞指示剂配制、基准液配制、碱液标定、甲醛滴定与酶解度计算。依据蛋白质酶解度的定义与相关计算公式，利用甲醛滴定法测定并计算出胰蛋白酶对底物BSA在本实验中的特定酶解条件下的酶解度为6.31。

Determination of Degree of Enzymatic Hydrolysis of BSA by Formol Titration Method

In this paper,the DEH（degree of enzymatic hydrolysis） of Bovine Serum Albumin（BSA） was tested and calculated with formol titration method.The conception of DEH was established and named and a determination method was developed based on formol titration.A correction coefficient was introduced to correct the determination results because of the weakness of formol titration.The values of correction coefficient of glycine and lysine in formol titration were 0.797 and 1.292,but it was 52.47 in BSA because of its abundant lateral chain groups.The α-amino group was different from ε-amino group in formol titration.According to the definition and the calculation formula of DEH,the enzymatic hydrolysis results of the BSA,which digested with trypsin in limited condition,were obtained by the formol titration method and the DEH was 22.7 in this research.