| 米曲氨基酰化酶的纯化及固定化酶法拆分D,L-丙氨酸 |
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| 引用本文： | 王晓平,刘一鸣,王丽娟,张红缨,张今. 米曲氨基酰化酶的纯化及固定化酶法拆分D,L-丙氨酸[J]. 吉林大学学报(理学版), 1998, 0(4) |
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| 作者姓名： | 王晓平 刘一鸣 王丽娟 张红缨 张今 |
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| 作者单位： | 吉林大学酶工程国家重点实验室 |
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| 摘 要： | 利用硫酸铵分级与双水相萃取结合的方法从米曲霉中提取米曲氨基酰化酶，并将其固定在ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－２５上，用于拆分Ｄ，Ｌ－丙氨酸，并对该酶及固定化酶的酶学性质进行研究
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| 关 键 词： | 氨基酰化酶，固定化，丙氨酸，酶法拆分 |
Purifies of the Aminoacylase and the Resolution of D,L Alanine by the Method of Immobilized Enzyme |
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| Wang Xiaoping,Liu Yiming,Wang Lijuan,Zhang Hongying,Zhang Jin. Purifies of the Aminoacylase and the Resolution of D,L Alanine by the Method of Immobilized Enzyme[J]. Journal of Jilin University: Sci Ed, 1998, 0(4) |
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| Authors: | Wang Xiaoping Liu Yiming Wang Lijuan Zhang Hongying Zhang Jin |
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| Abstract: | The aminoacylase(EC.3.5.1.14) was isolated and purified from Asperigillus oryzae No.3042 by the combination of ammonium sulfate fractionation and aqueous two phase system. The pure enzyme was immobilized on DEAE Sephadex A 25 to prepare immobilized enzyme, which was used on the optical resolution of D,L Alanine. In order to search better resolution condition, the enzymological properties of the native aminoacylase from Asperigillus oryzae 3042 and the immobilized aminoacylase were studied in this paper. |
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| Keywords: | aminoacylase immobilization alanine resolution |
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