| HIV相关性口腔白色念珠菌rDNA基因型研究 |
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| 引用本文： | 张云操,刘奇,武有聪,郭利军,申元英,白丽.HIV相关性口腔白色念珠菌rDNA基因型研究[J].大理学院学报,2011,10(8):24-26. |
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| 作者姓名： | 张云操 刘奇 武有聪 郭利军 申元英 白丽 |
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| 作者单位： | 大理学院基础医学院,云南大理,671000 |
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| 基金项目： | 国家自然科学基金资助项目(C30260005); 大理学院高层次人才基金资助项目(KY430440) |
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| 摘 要： | 目的:了解HIV感染人群和健康人群口腔白色念珠菌基因型的分布情况。方法:运用CA-INT特异性引物聚合酶链反应方法扩增白色念珠菌包含1类内含子的编码rRNA的25S rDNA区,根据扩增条带大小进行基因分型,并应用χ2检验对54株HIV感染人群和54株健康人群口腔白色念珠菌基因型别进行分析。结果:CA-INT特异性引物PCR法可将108株口腔白色念珠菌分为A、B和C型,以A型最多见,两组人群口腔白色念珠菌基因型分布基本相同,但HIV感染人群口腔白色念珠菌A型所占构成比高于健康人群,差别有统计学意义。结论:HIV感染人群和健康人群口腔白色念珠菌均具有基因多态性,CA-INT特异性引物PCR法是较好的口腔白色念珠菌种内菌株间鉴定的方法。
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| 关 键 词： | 白色念珠菌 HIV 聚合酶链反应r DNA基因型 |
rDNA Genotyping of Oral Candida albicans Isolated from HIV-infected Population |
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| ZHANG Yuncao,LIU Qi,WU Youcong,GUO Lijun,SHEN Yuanying,BAI Li.rDNA Genotyping of Oral Candida albicans Isolated from HIV-infected Population[J].Journal of Dali University,2011,10(8):24-26. |
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| Authors: | ZHANG Yuncao LIU Qi WU Youcong GUO Lijun SHEN Yuanying BAI Li |
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| Institution: | ZHANG Yuncao,LIU Qi,WU Youcong,GUO Lijun,SHEN Yuanying,BAI Li(School of Basic Medicine,Dali University,Dali,Yunnan 671000,China) |
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| Abstract: | Objective: To investigate the distribution of genotypes of oral Candida albicans isolated from HIV-related group and healthy carriers group.Methods: PCR with CA-INT primers was designed to amplify group 1 intron-containing region in 25S rDNA of oral Candida albicans,the different strains of oral Candida albicans were classified into genotypes on the basis of bands from amplicons.Then the genotypic distribution results of all oral Candida albicans were analyzed with SPSS 17.0 by chi-square test.Results: One ... |
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| Keywords: | Candida albicans HIV PCR rDNA genotyping |
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