| 赛若金对HepG2.2.15细胞株HBV复制的抑制作用 |
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| 引用本文： | 颜江华,颜青,欧阳丽娟,陈国良,吴晓鹭,苏文金. 赛若金对HepG2.2.15细胞株HBV复制的抑制作用[J]. 厦门大学学报(自然科学版), 2000, 39(4): 552-555 |
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| 作者姓名： | 颜江华 颜青 欧阳丽娟 陈国良 吴晓鹭 苏文金 |
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| 作者单位： | 1. 厦门大学抗癌研究中心,福建,厦门,361005
2. 厦门市中医院传染科,福建,厦门,361002 |
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| 基金项目： | 厦门市科委资助项目!（Z98809） |
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| 摘 要： | 以转染有ＨＢＶ的ＨｅｐＧ２．２．１５细胞株为实验模型，用ＭＴＴ法测定赛若金对２．２．１５细胞的生长抑制作用，流式细胞仪测定药物对２．２．１５细胞周期时相的改变，通过ＥＬＩＳＡ方法观察赛若金对２．２．１５细胞分泌ＨＢｅＡｇ及ＨＢｓＡｇ的影响，ＰＣＲ－Ｈｙｂ杂交法检测赛若金对２．２．１５细胞ＨＢＶ ＤＮＡ复制的抑制作用。结果显示，赛若金对转染乙肝病毒的２．２．１５细胞有生长抑制作用，抑制率随药物浓度呈
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| 关 键 词： | 赛若金 HBV 复制 抑制作用 HepG2细胞株 干扰素 |
Inhibition of Sinogen on HBV Replication of HepG2.2.15 Cell line in Vitro |
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| YAN Jing-hu,YAN Qing,OU YANG Li-juan,CHEN Guo-liang,WU Xian-lu,SU Wen-jin. Inhibition of Sinogen on HBV Replication of HepG2.2.15 Cell line in Vitro[J]. Journal of Xiamen University(Natural Science), 2000, 39(4): 552-555 |
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| Authors: | YAN Jing-hu YAN Qing OU YANG Li-juan CHEN Guo-liang WU Xian-lu SU Wen-jin |
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| Abstract: | HepG2. 2. 15 cell line, transfected with HBV DN,was used as an experiment mod- el. MTT assay was employed to detect the growth inhibition of 2. 2.15 cells treated by Sinogen. The change of the cell cycle was measured by flow cytometry technique (FCM). The effects of Sinogen on the secret HBeAg, HBsAg, as well as HBVDNA, were assayed by ELISA method and PCR-Hybridization, respectively. The results showed that the growth inhibition of 2. 2. 15 cells was observed after treatment of Sinogen. The inhibition rate was dose-dependent and reached maximum when the treated concentration reached 3 200 IU/mL. Its IC50 was 4 078. 5 IU/mL. FCM assay showed that 2.2.15 cell's cycle was arrested on G1 and G2-M phage. The HBsAg secreting was inhibited by 90%, in the meantime, HBeAg and HBV DNA were partially inhibited by Sinogen. Their inhibition rates were 50% and 60%, respectively. |
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| Keywords: | IFN HBV HepG2.2.15 |
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