十种热带雨林植物光活化抗微生物活性的筛选

为从植物中寻找具有光调节生物活性的物质,通过滤纸片法和薄层色谱自显影技术,以金黄色葡菌球菌（Staphylococcus aureus）、枯草芽胞杆菌（Bacillus subtilis）、草分枝杆菌（Mycobacteium phlei）、环状芽胞杆菌（Bacillus circulans）为供试菌,对10种热带雨林植物进行光活化抗微生物活性的筛选和活性部位的确定.结果表明：0.1mg的狗花椒（Y10）氯仿提取物对枯草芽胞杆菌具有明显的光活化抗微生物活性,在紫外光照射后和黑暗中抑菌圈相差4mm,薄层色谱中0.5mg Y10氯仿提取物抗枯草芽胞杆菌的抑菌圈最大.本研究为进一步从该植物中寻找新型光敏剂奠定了基础.

Photo-Activitied Antimicrobial Activities Screening of 10 Tropical Rain Forest Plants

To find the light-medidated biological active substances,10 tropical rain forest plants were screened by the paper disc method and thin-layer chromatography bioautography technique to test their antimicrobial activity Staphylococcus aureus,Bacillus subtilis,Mycobacteium phlei and Bacillus circulan were employed as samples.The results showed that 0.1mg of Zanthoxylum planispinum sieb.et Zucc.(Y10) chloroform extracts have obvious photo-activitied antimicrobial activities for Bacillus subtilis.The antibacterial circle difference between the ultra-violet light and darkness is 4mm.And 0.5mg Y10 chloroform extracts have maximum inhibition zone for Bacillus subtilis in thin-layer chromatography.Our study can be used for further investigation in novel photosensitizers.