| 海藻酸钠的提纯及海藻酸钙多孔支架的制备 |
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| 引用本文： | 刘杨,任力,季培红,王迎军.海藻酸钠的提纯及海藻酸钙多孔支架的制备[J].华南理工大学学报(自然科学版),2012,40(7):142-147. |
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| 作者姓名： | 刘杨 任力 季培红 王迎军 |
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| 作者单位： | 华南理工大学材料科学与工程学院//国家人体组织功能重建工程技术研究中心//广东省生物医学工程重点实验室,广东广州,510640 |
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| 基金项目： | 国家自然科学基金重点项目 |
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| 摘 要： | 采用丙酮沉淀法对海藻酸钠原料进行了提纯,对纯化后的海藻酸钠进行了红外光谱测试和凝胶渗透色谱测试,并测定了提纯前后海藻酸钠样品中剩余蛋白质的含量.此外,通过CaCl2-海藻酸钠、CaSO4/GDL-海藻酸钠和Ca-EDTA/GDL-海藻酸钠3种交联体系制备了多种海藻酸钙三维多孔支架材料.研究结果表明:由质量分数为2.5%～3.0%的海藻酸钠所制得的Ca-EDTA/GDL-海藻酸钙三维多孔支架材料中微孔分布均匀,比表面积较大,且具有较好的力学性能,该支架有可能用作组织工程和细胞工程中培养细胞的培养基.
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| 关 键 词： | 组织工程 多孔支架 海藻酸钙 海藻酸钠 |
Purification of Sodium Alginate and Preparation of Porous Calcium Alginate Scaffolds |
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| Liu Yang
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Ren Li
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Ji Pei-hong
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Wang Ying-jun.Purification of Sodium Alginate and Preparation of Porous Calcium Alginate Scaffolds[J].Journal of South China University of Technology(Natural Science Edition),2012,40(7):142-147. |
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| Authors: | Liu Yang Ren Li Ji Pei-hong Wang Ying-jun |
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| Institution: | Liu Yang Ren Li Ji Pei-hong Wang Ying-jun(School of Materials Science and Engineering∥National Engineering Research Center for Tissue Restoration and Reconstruction ∥Guangdong Province Key Laboratory of Biomedical Sciences and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China) |
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| Abstract: | Sodium alginate(AlgNa) was purified via the acetone precipitation,and the product was tested by means of FT-IR and GPC.Then,the contants of residual protein in both the unpurified and the purified samples were measured,and several three-dimension porous calcium alginate scaffolds were fabricated through such three crosslinking systems as CaCl2-AlgNa,CaSO4/GDL-AlgNa and Ca-EDTA/GDL-AlgNa.The results indicate that the porous Ca-EDTA/GDL-AlgNa scaffold with a sodium alginate content of 2.5% ～ 3.0% possesses uniform micropore distribution,large specific surface area and excellent mechanical behavior,which means that it is suitable for the cell culture in tissue engineering and cell engineering. |
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| Keywords: | tissue engineering porous scaffold calcium alginate sodium alginate |
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