| 丁酸钠对M期同步及非同步的HeLa细胞早G_1期阻断的可逆性 |
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| 引用本文： | 韩凤霞,张鸿卿,曾长青,彭安,薛绍白. 丁酸钠对M期同步及非同步的HeLa细胞早G_1期阻断的可逆性[J]. 北京师范大学学报(自然科学版), 1987, 0(1) |
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| 作者姓名： | 韩凤霞 张鸿卿 曾长青 彭安 薛绍白 |
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| 作者单位： | 北京师范大学生物系，北京师范大学生物系，北京师范大学生物系，北京师范大学生物系，北京师范大学生物系 进修教师 湖南医学院工作 |
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| 摘 要： | 本文采用早熟染色体凝集(PCC)和~3H-TdR显微自显影技术,观察丁酸钠对同步及非同步的HeLa细胞早G_1期阻断的可逆性及其可逆的进程。实验发现丁酸钠对HeLa细胞早G_1期阻断完全是可逆的,且这种可逆是在洗去丁酸钠后立即产生,丁酸钠对HeLa细胞早G_1期阻断的时间点发生在细胞进入S期前的9.5h;发现早G_1期阻断后的恢复必须再经过中、晚G_1期然后才能进入S期.
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| 关 键 词： | 丁酸钠 细胞周期 早熟染色体凝集 显微自显影术 |
REVERSIBILITY OF THE EARLY G_1 BLOCKING OF THE ASYNCHRONOUS AND M-SYNCHRONIZED HeLa CELLS TREATED BY N-BUTYRATE |
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| Han Fengxia,Zhang Hongqing,Zeng Changqing,Peng An and Xue Shaobai. REVERSIBILITY OF THE EARLY G_1 BLOCKING OF THE ASYNCHRONOUS AND M-SYNCHRONIZED HeLa CELLS TREATED BY N-BUTYRATE[J]. Journal of Beijing Normal University(Natural Science), 1987, 0(1) |
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| Authors: | Han Fengxia Zhang Hongqing Zeng Changqing Peng An Xue Shaobai |
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| Affiliation: | Department of Biology |
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| Abstract: | It is discovered that 5mmol butyrate blocks the asynchronous and M-phase synchronized HeLa cells in early G_1 phase. It seems that the deter- mined effects of butyrate are blocking and delaying the early G_1 phase cell to enter into the mid and late G_1 phase. Now the early G_1 reversible blocking effects of the n-butyrate is studied with PCC(Premature Chromosome Conden- sation) and microautoradiograph cell cycle analysis. Both of these results show that the early G_ blocking effect can be reversed after washing out of n-butyrate. The blocking site of n-butyrate is found at 4.5~5.5 h before the initiation of S phase. |
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| Keywords: | n-butyrate cell cycle premature chromosome condensition microautoradiography. |
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