| 大鼠骨髓基质细胞培养的实验研究 |
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| 引用本文： | 张昕.大鼠骨髓基质细胞培养的实验研究[J].北华大学学报(自然科学版),2010,11(1):43-45. |
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| 作者姓名： | 张昕 |
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| 作者单位： | 吉林市第二人民医院,吉林,吉林,132001 |
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| 摘 要： | 目的探讨利用骨髓基质细胞体外培养获得神经干细胞的方法及应用.方法体外培养大鼠骨髓基质细胞;免疫组化检测;流式细胞仪检测.结果加BME的骨髓基质细胞大量分裂,形成大量的细胞分裂球,传到4代时,90%以上的细胞表达Nestin阳性.结论BME对骨髓基质细胞有明显的促分裂作用,应用此种方法可获得神经干细胞(Neural stem cell,NSC).
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| 关 键 词： | 骨髓基质细胞 碱性成纤维细胞生长因子 分裂 |
Experimental Study and Identification of Rat Bone Marrow Stromal Cell Culture |
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| ZHANG Xin.Experimental Study and Identification of Rat Bone Marrow Stromal Cell Culture[J].Journal of Beihua University(Natural Science),2010,11(1):43-45. |
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| Authors: | ZHANG Xin |
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| Institution: | ZHANG Xin (The Second People\'s Hospital of Jilin City,Jilin 132001,China) |
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| Abstract: | ObjectiveThe neural stem cells(NSCs) cultured from bone marrow stromal cells in vitro were gained and identified.Method The rat bone marrow stromal cells in vitro were cultured and then detected by immunofluorescence staining and flow cytometer.ResultsA large number of bone marrow stromal cells with BME divided,formed a large number of cellular division spheres,more than 90% NSCs expressed positive Nestin after 4 generations.ConclusionBME can obviously promote the proliferation of marrow stromal cells,NSCs ... |
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| Keywords: | marrow stromal cells bFGF division |
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