| Apoptosis induced by （DIPP-L-Leu）2-L-Lys-OCH3 in K562 cells |
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| 引用本文： | NIUYanling JIANGYuyang CAOShengli DUWei ZHAOYufen. Apoptosis induced by （DIPP-L-Leu）2-L-Lys-OCH3 in K562 cells[J]. 科学通报(英文版), 2003, 48(9): 869-872. DOI: 10.1007/BF03325666 |
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| 作者姓名： | NIUYanling JIANGYuyang CAOShengli DUWei ZHAOYufen |
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| 作者单位： | [1]KeyLaboratoryofBioorganicPhosphorusChemistry,MinistryofEducation,DepartmentofChemistry,SchoolofLifeSciencesandEngineering,TsinghuaUniversity,Beijing100084,China [2]DepartmentofChemistry,CapitalNormalUniversity,Beijing100084,China |
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| 摘 要： | Apoptosis as a mechanism of deleting cells from tissues plays an important role in physiological and varieties of pathological situations, especially cancer conditions. In order to search for tumor cells apoptosis inducers, the inhibition effects on K562 cells of N-phosphoryl dipeptide methyl esters were studied by MTT assays, and (DIPP-L-Leu)2-L-Lys-OCH3 was the compound which had the best activity. From the studies of the typical apoptotic morphologic changes, DNA agarose gel electrophoresis, and flow cytometry analysis, it could be concluded that (DIPP-L-Leu)2-L-Lys-OCH3 could induce apoptosis of K562 cells in a dose-dependent manner, and the IC50 was 22.66 μmol/L according to MTT assays.
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| 关 键 词： | 细胞凋亡 细胞周期 K562细胞 N-磷酸化酶二肽甲基脂 |
| 收稿时间： | 2002-07-08 |
Apoptosis induced by (DIPP-L-Leu)2-L-Lys-OCH3 in K562 cells |
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| Yanling Niu,Yuyang Jiang,Shengli Cao,Wei Du,Yufen Zhao. Apoptosis induced by (DIPP-L-Leu)2-L-Lys-OCH3 in K562 cells[J]. Chinese science bulletin, 2003, 48(9): 869-872. DOI: 10.1007/BF03325666 |
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| Authors: | Yanling Niu Yuyang Jiang Shengli Cao Wei Du Yufen Zhao |
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| Affiliation: | e-mail: yuyang_jiang@yahoo. com. cn |
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| Abstract: | Apoptosis as a mechanism of deleting cells from tissues plays an important role in physiological and varieties of pathological situations, especially cancer conditions. In order to search for tumor cells apoptosis inducers, the inhibition effects on K562 cells of N-phosphoryl dipeptide methyl esters were studied by MTT assays, and (DIPP-L-Leu)2- L-Lys-OCH3 was the compound which had the best activity. From the studies of the typical apoptotic morphologic changes, DNA agarose gel electrophoresis, and flow cytometry analysis, it could be concluded that (DIPP-L-Leu)2- L-Lys-OCH3 could induce apoptosis of K562 cells in a dose-dependent manner, and the IC50 was 22.66 mol/L according to MTT assays. |
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| Keywords: | apoptosis cell cycle N-phosphoryl dipeptide methyl ester K562 cells. |
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