| 在康宁木霉诱变育种过程中环境物理参数对致死率的影响 |
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| 引用本文： | 孟庆云,张鹏,张永嘉. 在康宁木霉诱变育种过程中环境物理参数对致死率的影响[J]. 北京化工大学学报(自然科学版), 2000, 27(2): 80-83 |
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| 作者姓名： | 孟庆云 张鹏 张永嘉 |
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| 作者单位： | 1)北京化工大学应用数理系; 2)化学工程学院,北京 100029 |
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| 摘 要： | 报告了使用物理诱变剂 (紫外线)和化学诱变剂 (亚硝基胍)对康宁木霉进行诱变处理时,环境物理因素对微生物致死率的影响。实验表明,当诱变剂的剂量或作用时间增加时 (包括物理和化学诱变剂) ,微生物的致死率会明显上升;微生物经过物理或化学诱变剂的处理后,再将其放入非自然环境中培养 (包括在电磁场中处理;在屏蔽室中处理及在高γ本底辐射的情况下处理) ,其致死率会有所变化;在诱变时采用多种诱变剂,及在孢子悬液中添加微量元素和限量培养液是提高微生物致死率的另一种方法。
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| 关 键 词： | 致死率 诱变 环境物理参数 致死率 诱变 环境物理参数 |
| 收稿时间： | 1999-12-07 |
Influence of environmental physical parameters on the killing rate of Trichoderma Kongii in breeding |
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| MENG Qing yun ) ZHANG Peng ) ZHANG Yong jia ) WU Wen ge ). Influence of environmental physical parameters on the killing rate of Trichoderma Kongii in breeding[J]. Journal of Beijing University of Chemical Technology, 2000, 27(2): 80-83 |
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| Authors: | MENG Qing yun ) ZHANG Peng ) ZHANG Yong jia ) WU Wen ge ) |
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| Affiliation: | 1）Department of Applied Mathematics and Physics; 2）College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | This paper reports the influence environmental physical parameters on the killing rate of microbes in Trichoderma Kongii(13003) breeding with physical(ultraviolet ray) and chemical(NTG) mutagens. Experimental results show that the killing rate of microbes clearly goes up as the dose of mutation agent or action time increases. The killing rate will vary if the microbes are cultivated in a non natural environment(processed in an electromagnetic field, in a shielded room and in a high background of γ radiatio) after being induceced. Adding trace elment and limiting the quantity of cultivation liquid is anther way to increase the killing rate of the induce microbes. |
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| Keywords: | killing rate mutation physical parameters of environment |
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