| 脉冲电场对中国红豆杉细胞的影响 |
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| 引用本文： | 黄琳玲,叶 红,陈树德,钟建江. 脉冲电场对中国红豆杉细胞的影响[J]. 华东师范大学学报(自然科学版), 2006, 2006(4): 16-22 |
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| 作者姓名： | 黄琳玲 叶 红 陈树德 钟建江 |
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| 作者单位： | [1]华东师范大学光谱学与波谱学教育部重点实验室,华东师范大学物理系,上海200062 [2]华东理工大学生物反应器工程国家重点实验室,上海200237 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 通过二维电泳（2-DE）技术来研究脉冲电场作用后48 h中国红豆杉细胞在蛋白表达上的变化.共进行了3次二维电泳重复实验，经过相关因子分析证实具有较高的重复性.比较对照组和经过脉冲电场处理的实验组的二维电泳图谱，发现有较明显的差异.用基质辅助激光解析-飞行时间质谱和液相色谱-电喷雾质谱对其中的部分蛋白进行分析鉴定.鉴定结果表明：核糖核酸蛋白粒、醛糖差向异构酶和铁氧还蛋白-NADP(+)还原酶这三个蛋白可能是与脉冲电场诱导相关的蛋白.
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| 关 键 词： | 脉冲电场 中国红豆杉细胞 二维电泳 基质辅助激光解析-飞行时间质谱 液相色谱-电喷雾质谱 |
| 文章编号： | 1000-5641（2006）04-0016-07 |
| 收稿时间： | 2004-12-01 |
| 修稿时间： | 2004-12-01 |
Effect of Pulsed Electric Field on the Suspension Cultures of Taxus chinensis |
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| HUANG Lin-ling,YE Hong,CHEN Shu-de,ZHONG Jian-jiang. Effect of Pulsed Electric Field on the Suspension Cultures of Taxus chinensis[J]. Journal of East China Normal University(Natural Science), 2006, 2006(4): 16-22 |
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| Authors: | HUANG Lin-ling YE Hong CHEN Shu-de ZHONG Jian-jiang |
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| Affiliation: | 1. Key nology, Shanghai 200237, China |
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| Abstract: | Two dimensional gel electrophoresis(2-DE) was used to analyse changes of the protein ex- pression of suspension cultures of Taxus chinensis 48 hours after pulsed electric field(PEF)elicitation. The 2-DE experiments were repeated for 3 times,and proved to be reliable according to the correlation coefficient analysis.Distinct changes were found from comparison of the protein patterns of control and PEF treated cells.Matrix-assisted laser desorption/ionization mass spectrometry and electrospray ioni- zation mass spectrometry were used to analyse some proteins.The results indicated that heterogeneous nuclear ribonucleoprotein(hnRNP),aldose l-epimerase family protein and Ferredoxin-NADP( )re- ductase,putative/adrenodoxin reductase are related to the induction of PEF. |
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| Keywords: | pulsed electromagnetic field(PEF) Taxus chinensis two dimensional gel elec- trophoresis matrix-assisted laser desorption/ionization mass spectrometry electrospray ionization mass spectrometry |
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