Induction of lymphokine-activated killer cells from nude mouse spleen cells by interleukin-4 |
| |
Authors: | I. S. Tunru H. Suzuki M. Kobayashi |
| |
Affiliation: | (1) First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930-01 Toyama, (Japan) |
| |
Abstract: | The induction of lymphokine-activated killer (LAK) cells from natural killer (NK) lineage cells by interleukin-4 (IL-4) was studied in vitro. Activation of nude mouse spleen cells by IL-4 generated cytotoxic cells, capable of killing NK-sensitive as well as NK-resistant tumor cells. The induction of peak lytic activity was demonstrated after 3 days of culture with IL-4. Surface marker analysis indicated that the majority of precursor cells were aGM1+, Thy1–, and the majority of effector cells were aGM1+, Thy1+, suggesting that IL-4 induced LAK cells from nude mouse spleen cells were similar to those from normal mouse spleen cells. The induction of nude mouse LAK cells by IL-4 was partially inhibited by anti-IL-4 or anti-interferon (IFN)- , antibody, and it was further inhibited by the combination of two antibodies, suggesting that IFN- , production was associated with LAK induction of NK lineage cells by IL-4. |
| |
Keywords: | IL-4 LAK cells nude mouse |
本文献已被 SpringerLink 等数据库收录! |
|