KIF5C, a kinesin motor involved in apical trafficking of MDCK cells |
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Authors: | Ksenia Astanina Ralf Jacob |
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Institution: | (1) Department of Cell Biology and Cell Pathology, Philipps University of Marburg, Robert-Koch-Str. 6, 35033 Marburg, Germany; |
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Abstract: | Polarized traffic in epithelial cells depends on well-organized pathways that direct secretory cargo to the apical or basolateral
plasma membrane. In MDCK cells, apical trafficking can be further divided into a lipid raft-dependent and a raft-independent
route, which separate biosynthetic cargo in a post-Golgi endosomal compartment. We have now identified KIF5C as a kinesin
motor for apical trafficking of both raft-associated sucrase isomaltase and raft-independent neurotrophin receptor. KIF5C
was identified by mass spectrometry in vesicle enriched fractions and on immunoisolated post-Golgi vesicles carrying apical
cargo. The amount of vesicle-associated KIF5C was highest on material isolated directly after trans-Golgi network release
and declined thereafter. Altogether, our data suggest that KIF5C is involved in the passage of apical cargo molecules to a
post-Golgi endosomal compartment, where further segregation into distinct vesicle populations proceeds. |
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Keywords: | |
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