| 应用DOP—PCR与染色体涂染技术鉴别赤麂的Y染色体 |
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| 引用本文： | 单祥年,张悦,等.应用DOP—PCR与染色体涂染技术鉴别赤麂的Y染色体[J].南京师大学报,2001,24(2):71-74. |
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| 作者姓名： | 单祥年 张悦 |
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| 作者单位： | [1]南京师范大学生命科学学院，南京210097 [2]东南大学医学院生物教研室，南京210009 |
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| 基金项目： | 国家自然科学基金资助项目 (No .396 70 393) |
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| 摘 要： | 流式细胞仪分离小麂的Y染色体，并应用DOP－PCR扩增后标记作为探针，分别与雄性及雄性赤麂中期核型进行了染色体涂染，结果表明，只有雄性赤麂Y2染色体与探针有明显的杂交信号，说明只有Y2染色体与赤麂的性别决定相关，除除了相麂Y1染色体在性别决定中的作用。
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| 关 键 词： | 赤麂 性染色体 DOP－PCR 染色体涂染 |
| 文章编号： | 1001-4616(2001)02-0071-04 |
| 修稿时间： | 2000年9月15日 |
Identification of Y Chromosome of Munticus Muntjac Vaginalis by Chromosome Painting and DOP-PCR |
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| Shan Xiangnian ,Zhang Yue,Wang Yi,Liu Ningsheng,Lu Xiaoxuan Nie Wenhui,Yang Fengtang,Wang Jinhuan,Chen Yuzhe.Identification of Y Chromosome of Munticus Muntjac Vaginalis by Chromosome Painting and DOP-PCR[J].Journal of Nanjing Normal University(Natural Science Edition),2001,24(2):71-74. |
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| Authors: | Shan Xiangnian Zhang Yue Wang Yi Liu Ningsheng Lu Xiaoxuan Nie Wenhui Yang Fengtang Wang Jinhuan Chen Yuzhe |
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| Institution: | Shan Xiangnian 1,2,Zhang Yue2,Wang Yi2,Liu Ningsheng2,Lu Xiaoxuan2 Nie Wenhui3,Yang Fengtang3,Wang Jinhuan3,Chen Yuzhe3 |
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| Abstract: | The Y chromosome of M reevesi were sorted by fluorescence activated chromosome sorting and were amplified and labeled by DOP-PCR.Painted probes of M reevesi were hybridized onto metaphase spreads obtained from female and male of M m vaginali.Strong signals were detected only on Y 2 chromosome.That indicates Y 2 is the real sex chromosome in M m vaginalis. |
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| Keywords: | M m vaginalis sex chromosome DOP-PCR chromosome painting |
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