Purification of pig kidney diamine oxidase (histaminase) and in vivo effects on passive cutaneous anaphylaxis and histamine induced bronchoconstriction of guinea-pigs

Summary Histaminase (diamine oxidase) was purified from pig kidney using cadaverine-Sepharose affinity chromatography. Pretreatment of guinea-pigs with histaminase caused marked inhibition of the passive cutaneous anaphylaxis. The effect of histaminase on histamine induced bronchoconstriction in vivo was studied. Histaminase significantly reduced the response in guinea-pigs. Histaminase is effective as a potent antihistaminic agent in vivo.