p53基因在小鼠胚胎发育过程中的表达

以E9日龄至E14日龄昆明种正常小鼠胚胎为材料,利用质粒扩增的、地高辛标记的基因探针在组织切片上进行DNA-mRNA 分子原位杂交,研究了p53基因在小鼠胚胎发育过程中的表达.结果表明, p53基因不参与E9和E10日胚胎发育中的器官原基形成,参与器官的进一步分化成熟过程.这些器官主要有眼、脑、心、肺、脊柱和面颌骨,肝组织的发育与其无关; p53基因一方面参与胚胎发育中的细胞周期调控,另一方面也参与了某些与细胞周期无关的过程;不同的器官有不同的细胞周期调控机制.

Study of Gene Expression of p53 in Mice's Embryo Development

Using in situ hybridization technique with Dig labeled probe, the expression of p53 gene in mice's embryo development has been studied in this thesis, the results can be summarized as fellows: p53 gene does not take part in the formation of organ proimordia during the development of embryo of age 9 ds and 10 ds. It takes part in the further process of organ differentiation (concerned organs include eyes, brain, heart, lung, backbone and cranium viscerale, except liver). On the one hand, p53 gene takes part in the cell regulation during embryo development, and on the other hand, it also takes part in coming processes, which do not relate with cell cycle.There are different regulation mechanisms in the different organs.