An arachidonic acid-specific lipoxygenase from the gorgonian coralPseudoplexaura porosa |
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Authors: | D R Doerge M D Corbett |
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Institution: | (1) Food Science and Human Nutrition Department, Pesticide Research Laboratory, The University of Florida, 32611 Gainesville, Florida, USA |
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Abstract: | Summary A novel lipoxygenase was partially purified from the gorgonian coralPseudoplexaura porosa and was found to be specific for arachidonic acid. This soluble enzyme catalyzed the formation of 15-hydroperoxy-eicosatetraenoic acid (15-HPETE) from arachidonic acid.This investigation was supported by PHS grant No. CA32395 awarded by the National Cancer Institute and by PHS Research Carreer Development Award No. ES00120 to MDC awarded by the National Institute of Environmental Health Sciences, DHHS. The authors acknowledge Ms M. A. Russell and Dr D. G. Anderson of the University of Miami for assistance in the collection of gorgonian corals. Preliminary work was conducted at the University of Miami School of Marine and Atmospheric Science. This is journal series No. 3638 from the Florida Agricultural Experiment Station. |
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