Isolation ofosRACD gene encoding a small GTP-binding protein from rice |
| |
Authors: | Zhiyong Mi Shusheng Wang Naihu Wu |
| |
Institution: | (1)Institute of Developmental Biology, Chinese Academy of Sciences ,Beijing 100080 ,China;
(2)Academy of Life Sciences, Peking University ,Beijing 100871 ,China |
| |
Abstract: | Using an improved version of mRNA differential display technology, we have obtained a differentially displayed fragment RDP-8. Homologous comparison indicated that the fragment RDP-8 has high homology with the gene encoding maize small GTP-binding protein. By screening cDNA library of the rice Nongken 58N pan icle using the newly obtained fragment RDP-8 as probe, we further found the full-length cDNA of osRACD gene that encodes a rice small GTP-binding protein. Asco mpared with maize RACD gene, the osRACD of rice shows remarkable homology in both nucleotide sequence and amino acid sequence, 88% and 97% respectively. Evidence from RT-PCR study indicates that osRACD gene is related to photoperiod fertility conversion of photoperiod sensitive genic male sterility (PSGMS) rice. |
| |
Keywords: | rice osRACD mRNA differential display light signal transduction |
本文献已被 万方数据 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |