| 黑胸大蠊免疫兔血清的制备与应用 |
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| 引用本文： | 吴仕筠,汪世平,徐绍锐,钟飞,李文凯. 黑胸大蠊免疫兔血清的制备与应用[J]. 吉首大学学报(自然科学版), 2008, 29(5): 104-107 |
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| 作者姓名： | 吴仕筠 汪世平 徐绍锐 钟飞 李文凯 |
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| 作者单位： | 吉首大学医学院,湖南,吉首,416000;吉首大学医学院,湖南,吉首,416000;中南大学湘雅基础医学院病原生物学系,湖南,长沙,410078;中南大学湘雅基础医学院病原生物学系,湖南,长沙,410078 |
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| 基金项目： | 湖南省科技厅科技计划重大专项，湖南省教育厅科学研究项目，湖南省卫生厅科研基金项目，湘西自治州指导性科技计划项目(2006CXZ08)基金联合资助 |
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| 摘 要： | 按常规动物免疫方法用黑胸大蠊不同发育阶段可溶性抗原对新西兰兔进行免疫，并通过SDS-PAGE和酶联免疫印迹(EL IB) 技术分析黑胸大蠊不同发育阶段抗原的免疫学特性，同时用黑胸大蠊若虫和雄虫免疫兔血清筛选美洲大蠊若虫cDNA 文库，对阳性克隆进行PCR 鉴定和序列测定，结果显示：卵抗原、若虫抗原、雄成虫抗原、雌成虫抗原分别可见13，28，26 和41 条蛋白区带，4种抗原组分相互之间有交叉抗原存在，用黑胸大蠊若虫免疫兔血清筛选出1个新基因，用黑胸大蠊雄虫免疫兔血清筛选出6个新基因.
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| 关 键 词： | 黑胸大蠊 免疫兔血清 制备 ELIB cDNA文库筛选 |
Preparation and Application of Periplaneta Fulignosa Imunized Rabbit Sera |
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| WU Shi-jun,WANG Shi-ping,XU Shao-rui,ZHONG Fei,LI Wen-kai. Preparation and Application of Periplaneta Fulignosa Imunized Rabbit Sera[J]. Journal of Jishou University(Natural Science Edition), 2008, 29(5): 104-107 |
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| Authors: | WU Shi-jun WANG Shi-ping XU Shao-rui ZHONG Fei LI Wen-kai |
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| Affiliation: | ( 1.Medical College,Jishou University,Jishou 416000,Hunan China;2.Department of Parasitology,Xiangya School of Medicine,Central South University,Changsha 410078,China) |
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| Abstract: | Soluble protein antigens from different developmental stages of Periplaneta fuliginosa were used to immunize rabbits.The immunological characteristics of antigens from different stages of Periplaneta fuliginosa were analyzed with SDS-PAGE and enzyme-linked immuno-blotting (ELIB).Rabbits anti-sera against nymph and adult antigens were used to screen the cDNA library of Periplaneta Americana nymph,and the positive clones were identified by PCR.Results showed that the egg antigens,nympha antigens,male and female adult antigens revealed 13,28,26,41 bands,respectively.The results also showed that there were cross-reactive antigen components among these groups.One and 6 new genes were screened with nympha and adult antigen immunized rabbit sera,respectively. |
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| Keywords: | ELIB |
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