| 微生物发酵转化芦丁制备槲皮素的研究 |
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| 引用本文： | 高陪,苏建,沈竞,孙启玲.微生物发酵转化芦丁制备槲皮素的研究[J].四川大学学报(自然科学版),2010,47(6):1429-1433. |
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| 作者姓名： | 高陪 苏建 沈竞 孙启玲 |
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| 作者单位： | 四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都,610065 |
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| 基金项目： | 成都市科技局项目(ZGGYB017NC-010) |
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| 摘 要： | 用本实验室筛选得到的一株黑曲霉以芦丁为底物进行液态发酵,以达到将芦丁转化为槲皮素的目的.采用正交实验法对液态发酵培养基及发酵条件进行优化,得到最佳发酵条件为:芦丁3%,NaNO_3 0.3%,K_2HPO_40.1%,MgSO_40.05%,KCl0.05%,FeSO_40.01%;pH=6;装液量20%,接种量20%,培养条件为35℃,200 r/min.在此条件下,芦丁的转化率为97.78%,槲皮素的得率为90.39%.采用正交实验法优化槲皮素的提取条件,得到最佳提取条件为酒精浓度60%,料水比1:6,温度60℃,提取4次,每次60min.在此条件下,槲皮素的提取率为95.37%.对提取得到槲皮素粗品进行热水沉淀,最终得到纯度为97.12%的槲皮素.
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| 关 键 词： | 微生物转化 芦丁 槲皮素 正交实验 |
The preparation of quercetin through the transformation from rutin by the microbial fermentation |
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| GAO Pei,SU Jian,SHEN Jing,SUN Qi-Ling.The preparation of quercetin through the transformation from rutin by the microbial fermentation[J].Journal of Sichuan University (Natural Science Edition),2010,47(6):1429-1433. |
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| Authors: | GAO Pei SU Jian SHEN Jing SUN Qi-Ling |
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| Institution: | Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences,Sichuan University;Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences,Sichuan University;Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences,Sichuan University;Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences,Sichuan University |
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| Abstract: | |
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| Keywords: | microbial transformation rutin quercetin orthogonal layout |
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