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| 反相高效液相色谱法测定家兔粪便中芒果苷的含量 | |
| 引用本文： | 曾春晖,潘小姣,杨柯,唐德智,邓家刚,樊立勇.反相高效液相色谱法测定家兔粪便中芒果苷的含量[J].科学技术与工程,2009,9(9). |
| 作者姓名： | 曾春晖 潘小姣 杨柯 唐德智 邓家刚 樊立勇 |
| 作者单位： | 1. 广西中医学院药学院,南宁,530001 2. 广西南宁市食品药品检验所,南宁,530001 3. 广西三特医药有限公司,南宁,530011 |
| 基金项目： | 广西自治区科学基金，广西高校人才小高地建设创新团队计划，国家科技部西部开发重大项目 |
| 摘 要： | 为研究家兔一次口服芒果苷后芒果苷在粪便中的排泄情况,应用反相高效液相色谱法测定芒果苷给予家兔口服后,第1～6 d粪便中芒果苷的含量.家兔口服芒果苷后第1～2 d可在粪便中测出原型芒果苷,第1 d的排出率为20.321 7%,第2 d的排出率为4.118 3%,第3 d以后在粪便中不再检测出原型芒果苷,芒果苷粪便总排出率为24.44%;芒果苷含测方法的回收率为99.87%,RSD为1.92%.说明本实验建立的反相高效液相色谱法测定粪便中芒果苷浓度方法准确灵敏、重现性好.芒果苷经家兔一次性口服后,约有25%通过粪便以原型形式排泄. |
| 关 键 词： | 芒果苷 反相高效液相色谱法 家兔 粪便 |
Determination of Mangiferin in Dejecta of Rabbits by RP-HPLC |
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| ZENG Chun-hui,PAN Xiao-jiao,YANG Kang,TANG De-zhi,DENG Jia-gang,FAN Li-yong.Determination of Mangiferin in Dejecta of Rabbits by RP-HPLC[J].Science Technology and Engineering,2009,9(9). | |
| Authors: | ZENG Chun-hui PAN Xiao-jiao YANG Kang TANG De-zhi DENG Jia-gang FAN Li-yong |
| Institution: | Pharmacy College of Guangxi TCM University;Nanning 530001;P.R.China;Nanning Institute for Food and Drug Control1;Guangxi Sante Medical and Pharmaceutical Co.;Ltd2;Nanning 530011;P.R.China |
| Abstract: | To investigate the excretion process of mangiferin after single oral administration in rabbit,RP-HPLC was used to determinate the mangiferin in dejecta of rabbits in 6 days after administration.It is resulted that the prototype of mangifein was detected in the first day and the second day after administration,instead of the third day.The fecal excretion rates were 20.321%,4.1183% respectively,and the total fecal excretion rate was 24.44%.The recovery rate of mangiferin was 99.87%,RSD was 1.92%.It is conclus... |
| Keywords: | mangiferin RP-HPLC rabbit dejecta |
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