| 分光光度法测定酸性粘多糖 |
| |
| 引用本文： | 孙瑞叶,高华,刘坤. 分光光度法测定酸性粘多糖[J]. 青岛大学学报(自然科学版), 2002, 15(3): 21-23 |
| |
| 作者姓名： | 孙瑞叶 高华 刘坤 |
| |
| 作者单位： | 1. 青岛第十三中学,山东,青岛,266012
2. 青岛大学医学院,山东,青岛,266021 |
| |
| 摘 要： | 建立一种快速准确的动物粘多糖测定方法。利用含有苯三酚的酸性溶液与样品中的粘多糖反应生成有色产物进行分光光度分析。558nm波长处测定粘多糖质量浓度在0.10-1.0g/L范围内符合比耳定律，并具有较高的准确度和精密度。
|
| 关 键 词： | 酸性粘多糖 分光光度法 苯三酚 含量测定 动物多糖 天然药用活性成分 |
| 文章编号： | 1006-1037(2002)03-0021-03 |
| 修稿时间： | 2002-05-22 |
SPECRTOPHOTOMETRIC DETERMINATION OF MUCOPOLYSACCHARIDE WITH PHLORGLUCINOL |
| |
| SUN Ruiye ,GAO Hua ,LIU Kun. SPECRTOPHOTOMETRIC DETERMINATION OF MUCOPOLYSACCHARIDE WITH PHLORGLUCINOL[J]. Journal of Qingdao University(Natural Science Edition), 2002, 15(3): 21-23 |
| |
| Authors: | SUN Ruiye GAO Hua LIU Kun |
| |
| Affiliation: | SUN Ruiye 1,GAO Hua 2,LIU Kun 2 |
| |
| Abstract: | To establish a rapid and precise method for the determination of mucopolysaccharide,the samples of mucopolysaccharide were determined with spectrophotometry, based on the reaction of mucopolysaccharide with a mineral acid containing phlorglucinol solution. Absorbance value at 558 nm obey Beer's Law within the concentration range from 0.10 to 1.0 g/L, and the results were precise and accurate. The method is operationally simple and has a good precision and an adequate selectivity. |
| |
| Keywords: | mucopolysaccharide spectrophotometry phlorglucinol |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
