THE蛋白的结构分析与功能预测

调用motif数据库、profile数据库和interproscan数据库,对THE蛋白进行了序列同源性分析和功能位点分析.结果表明,THE蛋白质是一种核蛋白,理论等电点为6.36,分子量为44 859 Dalton.应用多种相关软件对THE蛋白的二级结构和特殊结构进行了初步预测,结果显示:THE蛋白中存在α螺旋、β-折叠片和无规卷曲结构,有两个可形成跨膜结构的片段,不存在卷曲螺旋,无信号肽,也无线粒体定位信号.对THE蛋白进行序列同源性、结构域及功能位点预测,结果显示:THE蛋白与来自大鼠睾丸的Tes13-S、Fos13-L和几种假设蛋白有较高的相似性;THE蛋白存在次黄嘌呤核苷酸脱氢酶、嘌呤核苷酸还原酶结构域及PKC、酰胺化、豆蔻酸连接等功能位点,无糖基化位点.THE蛋白的结构分析与功能预测为该基因的功能研究提供了重要的依据.

Structure Analysis and Function Prediction of THE Protein

THE(estis High Expression Gene) is a new gene produced by e-cloning which has a high expression in human testis.The ORF(open reading frame) of THE encode 388 amino acids.In the study,several softwares were used to predict Secondary Structure and Special Structure of THE protein,as a result: THE protein has Alpha helix,Extended strand,Random coil structure and two possible transmembrane helices,while no COILS,Signal peptide and Mitochondria target peptide exist in this sequence.The sequence homology,domains and functional sites of THE protein are also predicted in this paper,and the results are: THE protein has high identity score with several hypothetical proteins and Tes13-S,Fos13-L derived from testis of mouse;IMP dehydrogenase / GMP reductase domain,Protein Kinase C phosphorylation site,N-myristoylation site,Amidation site and other functional sites were found in THE protein.