| 龙葵碱对HepG2细胞磷脂酰丝氨酸及断裂DNA研究 |
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| 引用本文： | 季宇彬,徐丽丽,高世勇.龙葵碱对HepG2细胞磷脂酰丝氨酸及断裂DNA研究[J].哈尔滨商业大学学报(自然科学版),2008,24(3):268-273. |
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| 作者姓名： | 季宇彬 徐丽丽 高世勇 |
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| 作者单位： | 哈尔滨商业大学,生命科学与环境科学研究中心药物研究所,博士后科研工作站,哈尔滨150076;国家教育部,抗肿瘤天然药物工程研究中心,哈尔滨,150076 |
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| 基金项目： | 国家自然科学基金
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黑龙江省自然科学基金
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黑龙江省哈尔滨市青年科学基金 |
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| 摘 要： | 研究龙葵碱对肝癌细胞HepG2的诱导凋亡作用.采用倒置显微镜观察形态学变化,Annexin V-FITC联合PI双染法和Tunel法检测龙葵碱诱导人肝癌HepG2细胞凋亡作用.结果发现龙葵碱作用24 h后,贴壁细胞数量减少、体积缩小,变圆等明显凋亡形态;在激光共聚焦显微镜下观察到AnnexinV-FITC阳性信号为绿色荧光,PI为红色荧光;Tunel阳性信号为绿色荧光.表明龙葵碱通过诱导HepG2细胞凋亡达到抗肿瘤的作用.
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| 关 键 词： | 龙葵碱 细胞凋亡 Annexin V-FITC联合PI双染 TUNEL |
| 文章编号： | 1672-0946(2008)03-0268-06 |
| 修稿时间： | 2007年9月12日 |
Study on PS and cracking DNA in HepG2 cells induced by solanine |
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| JI Yu-bin,XU Li-li,GAO Shi-yong.Study on PS and cracking DNA in HepG2 cells induced by solanine[J].Journal of Harbin University of Commerce :Natural Sciences Edition,2008,24(3):268-273. |
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| Authors: | JI Yu-bin XU Li-li GAO Shi-yong |
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| Institution: | JI Yu-bin, XU Li-li, GAO Shi-yong( 1. Postdoctoral Research Station of Institute of Medicine of Center of Research and Development on Life and Environment Science, Harbin University of Commerce, Harbin 150076, China;2. Engineering Research Center of Natural Anticancer Drugs , Ministry of Education; Harbin 150076,China) |
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| Abstract: | To investigate the apoptosis effects of solanine in HepG2 cell. The morphology of cells is observed under transmission electron microscopy. Apoptosis of solanine is assessed with Annexin V - FITC/PI and Tunel. The result is that with the inverted microscope, after being treated with drug-solanine , cells grew against the wall and peeled off gradually, which showed the characters of cells apoptosis. With the laser confocal microscopic, the positive signal of Annexin V - FITC is green, and PI is red ; the positive signal of tunnel is green. Inhibition on tumor of solanine can be accomplished through inducing the apoptosis in HepG2 cells. |
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| Keywords: | solanine apoptosis annexin V - FITC/PI tunel |
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