| 结核分枝杆菌特异性抗原的N末端氨基酸序列分析 |
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| 引用本文： | 刘文,陈佩丽.结核分枝杆菌特异性抗原的N末端氨基酸序列分析[J].复旦学报(自然科学版),1998,37(4):532-536. |
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| 作者姓名： | 刘文 陈佩丽 |
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| 作者单位： | 复旦大学(刘文，陈佩丽，高大操，王洪海)，北京解放军309医院(庄玉辉)，上海生物制品所(陈岳祥)，上海第一肺科医院(桂晓红) |
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| 摘 要： | 把ＷｅｓｔｅｒｎＢｌｏｔ分析抗原抗体反应的高度特异性与ＰＶＤＦ膜作为载体进行蛋白质氨基酸序列分析的高度敏感性相结合对结核分枝菌的特异性抗原表位刊物筛选和分析鉴定，获得了一个分子质量为３１ｋｕ，Ｎ末端为ＡｌａＧｌｕＶａｌＡｓｐＬｅｕＶａｌＰｈｅＡｌａＶａｌＳｅｒＴｒｐＰｒｏＶａｌＧｌｙ的结核分枝杆菌抗原。
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| 关 键 词： | 结核分枝杆菌 抗原表位 序列分析 结核病 氨基酸 |
N-terminal Amino Acid Sequence Analysis of Specific Antigen of Mycobacterium Tuberculosis |
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| Liu Wen, Chen Peili, Gao Dacao, Zhuang Yuhui, Wang Honghai.N-terminal Amino Acid Sequence Analysis of Specific Antigen of Mycobacterium Tuberculosis[J].Journal of Fudan University(Natural Science),1998,37(4):532-536. |
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| Authors: | Liu Wen Chen Peili Gao Dacao Zhuang Yuhui Wang Honghai |
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| Abstract: | A specific 31 ku antigen of Mycobacterium tuberculosis was selected. and determined in combination of the high specificity of antigen-antibody reaction with the high sensitivity of amino acid sequence analysis on PVDF membrane. N-terminal sequence of the antigen is Ala Gin Val Asp Trp Leu Val Phe Ala Val Ser Trp Pro Val Gly. It has positive reaction with anti-M. tuberculosis MAb and with serum of TB patients. Selection of specific and protective antigens is the basis of tuberculosis diagnosis, theraphy and vaccine design. |
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| Keywords: | Mycobacterium tuberculosis antigen sequence analysis |
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