| Involvement of a cytoplasmic glyceraldehyde-3- phosphate dehydrogenase GapC-2 in low-phosphate- induced anthocyanin accumulation in Arabidopsis |
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| 引用本文： | WANG XiYao CHEN YiFang ZOU JunJie WU WeiHua. Involvement of a cytoplasmic glyceraldehyde-3- phosphate dehydrogenase GapC-2 in low-phosphate- induced anthocyanin accumulation in Arabidopsis[J]. 科学通报(英文版), 2007, 52(13): 1764-1770. DOI: 10.1007/s11434-007-0277-y |
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| 作者姓名： | WANG XiYao CHEN YiFang ZOU JunJie WU WeiHua |
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| 作者单位： | State Key Laboratory of Plant Physiology and Biochemistry （SKLPPB）, College of Biological Sciences, National Plant Gene Re-search Centre （Beijing）, China Agricultural University, Beijing 100094, China |
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| 基金项目： | Supported by the Chinese National Key Basic Research Project （Grant No. 2006CB 100100） |
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| 摘 要： | Phosphorous is one of the essential mineral elements for plant growth and development.Typically, the shoots of plant seedlings usually turn a dark-brown or purple colour under low-Pi stress. Using protein 2-D gel and peptide mass fingerprinting mapping (PMF) methods, a cytoplasmic glyceralde-hyde-3-phosphate dehydrogenase GapC-2 was identified as a low-Pi responsive protein in Arabidopsisplants. Expression of AtGapC-2 protein was significantly decreased after 4 d of low-Pi stress. Two in-dependent T-DNA insertion lines of GapC-2 gene (At1g13440) showed a hypersensitive phenotype inresponse to low-Pi stress compared with wild type plants, while the transgenic complementation linesof the mutants showed a similar phenotype to the wild type. These results indicate that AtGapC-2 mayplay an important role in Arabidopsis responses to low-Pi stress, possibly by regulation of glycoly-sis-associated "Pi-pool" and accumulation of anthocyanin pigments in plants.
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| 关 键 词： | 阿布属植物 甘油醛-3-磷酸脱氢酶 电泳实验 突变体 |
| 收稿时间： | 2007-05-23 |
| 修稿时间： | 2007-05-23 |
Involvement of a cytoplasmic glyceraldehyde-3-phosphate dehydrogenase GapC-2 in low-phosphate-induced anthocyanin accumulation in Arabidopsis |
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| Wang XiYao,Chen YiFang,Zou JunJie,Wu WeiHua. Involvement of a cytoplasmic glyceraldehyde-3-phosphate dehydrogenase GapC-2 in low-phosphate-induced anthocyanin accumulation in Arabidopsis[J]. Chinese science bulletin, 2007, 52(13): 1764-1770. DOI: 10.1007/s11434-007-0277-y |
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| Authors: | Wang XiYao Chen YiFang Zou JunJie Wu WeiHua |
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| Affiliation: | (1) State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing, 100094, China |
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| Abstract: | Phosphorous is one of the essential mineral elements for plant growth and development. Typically, the shoots of plant seedlings usually turn a dark-brown or purple colour under low-Pi stress. Using protein 2-D gel and peptide mass fingerprinting mapping (PMF) methods, a cytoplasmic glyceralde-hyde-3-phosphate dehydrogenase GapC-2 was identified as a low-Pi responsive protein in Arabidopsis plants. Expression of AtGapC-2 protein was significantly decreased after 4 d of low-Pi stress. Two in-dependent T-DNA insertion lines of GapC-2 gene (At1g13440) showed a hypersensitive phenotype in response to low-Pi stress compared with wild type plants, while the transgenic complementation lines of the mutants showed a similar phenotype to the wild type. These results indicate that AtGapC-2 may play an important role in Arabidopsis responses to low-Pi stress, possibly by regulation of glycoly-sis-associated "Pi-pool" and accumulation of anthocyanin pigments in plants. |
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| Keywords: | Arabidopsis thaliana glyceraldehyde-3-phosphate dehydrogenase low-phosphorous stress 2-D gel electrophoresis T-DNA insertion mutant |
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