Multiple transgenes Populus xeuramericana 'Guariento' plants obtained by biolistic bombardment |
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作者姓名: | Wang JianGe Su XiaoHua Ji LiLi Zhang BingYu Hu ZanMin Huang RongFeng Tian YingChuan |
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作者单位: | [1]Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China [2]College of Landscape and art, Jiangxi Agricultural University, Nanchang 330045, China [3]Institute of Genetics and developmental biology, Chinese Academy of Sciences, Beijing 100101, China [4]Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China [5]Microbiology Institute, Chinese Academy of Sciences, Beijing 100080, China |
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基金项目: | The authors would like to thank Dr. Hu Jun for Southern blotting. |
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摘 要: | A JERF36 regulation gene, a selection marker gene (NPT-Ⅱ), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana 'Guariento' using biolistic bombardment; 25 kanamycin resistant plants were obtained, The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P, xeuramericana 'Guariento' and 5 genes were all transferred into 7 poplar plants, The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land,
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关 键 词: | 森林 生态系统 全球变暖 二氧化碳 植被 |
收稿时间: | 2006-06-08 |
修稿时间: | 2006-06-082006-11-02 |
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