| 重组人成纤维细胞生长因子21对胡萝卜遗传转化的研究 |
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| 引用本文： | 汪洪,蔡进章,曾建军.重组人成纤维细胞生长因子21对胡萝卜遗传转化的研究[J].井冈山大学学报（自然科学版）,2016(1):57-61. |
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| 作者姓名： | 汪洪 蔡进章 曾建军 |
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| 作者单位： | 温州医科大学药学院, 浙江, 温州 325035,温州医科大学附属第二医院, 浙江, 温州 325000,井冈山大学生命科学学院, 江西, 吉安 343009 |
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| 基金项目： | 浙江省自然科学基金项目(Y3100459) |
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| 摘 要： | 人成纤维细胞生长因子21(FGF21)是肝脏合成的一种可分泌多肽,对糖脂代谢的调控具有重要作用。以胡萝卜(Daucus carota L.)七寸人参品种为试验材料,用含重组人成纤维细胞生长因子21(rh FGF21)质粒p1390RⅡ的根癌农杆菌LBA4404对胡萝卜进行了遗传转化实验。PCR扩增和Western blot结果表明外源基因FGF21已成功转入胡萝卜基因组中。此研究为今后用胡萝卜产业化表达生产FGF21提供参考。
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| 关 键 词： | FGF 21 胡萝卜 农杆菌 遗传转化 |
| 收稿时间： | 2015/10/7 0:00:00 |
| 修稿时间： | 2015/12/17 0:00:00 |
GENETIC TRANSFORMATION OF RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR 21 (hFGF21) ON DAUCUS CAROTA L. |
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| WANG Hong,CAI Jin-zhang and ZENG Jian-jun.GENETIC TRANSFORMATION OF RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR 21 (hFGF21) ON DAUCUS CAROTA L.[J].Journal of Jinggangshan University(Natural Sciences Edition),2016(1):57-61. |
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| Authors: | WANG Hong CAI Jin-zhang and ZENG Jian-jun |
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| Institution: | School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035 China,The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325035 China and College of Life Sciences, Jinggangshan University, Ji'an, Jiangxi 343009, China |
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| Abstract: | Human fibroblast growth factor 21 (hFGF21), found in the mouse embryo, is a new member of fibroblast growth factor family. It is synthesized in the liver as secreting protein. In this study, Daucus carota L. hypocotyl was used as experimental materials. Recombinant human fibroblast growth factor 21 (rhFGF21) was introduced into D. carota via Agrobacterium tumefaciens-mediated method. The regenerated hygmycin resistant plantlets were confirmed by PCR and Western blotting. The results provided an experimental basis for large-scale production of FGF21. |
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| Keywords: | hFGF21 Daucus carota L Agrobacterium tumefaciens genetic transformation |
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