| 从福尔马林固定的癌组织中提取RNA的方法 |
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| 引用本文： | 邓昊,靳楠,张磊,刘丽江.从福尔马林固定的癌组织中提取RNA的方法[J].江汉大学学报(自然科学版),2010,38(4):85-89. |
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| 作者姓名： | 邓昊 靳楠 张磊 刘丽江 |
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| 作者单位： | [1]江汉大学医学院病理学与病理生理学教研室,湖北武汉430056 [2]江大病理诊断所,湖北武汉430056 |
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| 基金项目： | 国家自然科学基金面上项目，湖北省自然科学基金，武汉市青年科技晨光计划项目 |
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| 摘 要： | 目的:寻求从福尔马林固定的癌组织中提取RNA的合理方法.方法:运用Trizol法从福尔马林固定的胃癌组织中提取RNA,用分光光度计测定RNA的产量和纯度;通过RT-PCR对不同长度的管家基因-actin进行扩增.结果:100 bp和300 bp的-actin均能顺利扩增出特异性条带,500 bp的-actin未能扩增.结论:用Trizol法从福尔马林固定的癌组织中提取RNA是可行的;进行RT-PCR时应尽量设计小片段引物(100~300 bp)以获得更好的扩增效果.
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| 关 键 词： | 癌组织 福尔马林 固定 RT-PCR Trizol法 |
A Method for RNA Extraction from Formalin-fixed Cancer Tissue |
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| DENG Hao,JIN Nan,ZHANG Lei,LIU Li-jiang.A Method for RNA Extraction from Formalin-fixed Cancer Tissue[J].Journal of Jianghan University:Natural Sciences,2010,38(4):85-89. |
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| Authors: | DENG Hao JIN Nan ZHANG Lei LIU Li-jiang |
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| Institution: | 1.Department of Pathology and Pathophysiology,School of Medicine,Jianghan University,Wuhan 430056,Hubei,China;2.Jiangda Pathology Center,Jianghan University,Wuhan 430056,Hubei,China) |
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| Abstract: | Objective：To find a reasonable method for RNA extraction from formalin-fixed cancer tissue.Methods：To extract RNA from the formalin-fixed gastric cancer tissue with Trizol method.RNA yield and purity were detected with spectrophotometer,and RNA quality were de-tected by RT-PCR of different length housekeeping gene-actin.Results：High purity RNA can be extracted from the formalin-fixed gastric cancer tissue with this method.RT-PCR results showed that the 100 bp and 300 bp-actin can be successfully amplified,and the 500 bp-actin failed to am-plify specific bands.Conclusion：With Trizol method extracting RNA from formalin-fixed cancer tissue is feasible.We should try to design the small fragments primers（100-300bp） in order to obtain a higher amplification efficiency while carrying on RT-PCR. |
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| Keywords: | cancer tissue formalin fixed RT-PCR Trizol method |
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